iLite IL-12 Assay Ready Cells

$1,240.00

The iLite IL-12 Assay Ready Cells are designed for the specific detection of drug potency in serum/plasma as well as neutralizing antibodies. They utilize an assay technique called reporter gene assay ( RPG ) to analyze serum/plasma samples. The reporter genes utilized are encoded with a bioluminescent luciferase (Firefly luciferase). Different levels of luminescence detected from each reporter gene cell indicates different levels of expression. The iLite IL-12 Assay Ready Cells are for research use only and not for use in diagnostic or therapeutic procedures.

iLite IL-12 Assay Ready Cells

iLite IL-12 Assay Ready Cells Developed and Manufactured by Svar Life Science

The Eagle Biosciences iLite® IL-12 Assay Ready Cells are intended for:

  • Testing IL-12 or p40 inhibitor activity
  • Testing NAb response against IL-12

For Research Use Only


Key benefits of iLite® Assays


  • Highly specific reporter gene cell lines
  • Very sensitive cell line responses (>10 fold inductions)
  • Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
  • Assays within a workday (typically 4-7 hour assays)
  • Normalization gene, which eliminates unwanted matrix effects

Assay Background

Interleukin 12 (IL-12) is a 70 kDa cytokine produced principally by macrophages, neutrophils and dendritic cells in response to stimulation by antigen. IL-12 is a T cell-stimulating factor, playing a critical role in the regulation of T-helper 1 cell responses. IL-12 is composed of two subunits, p35 and p40, covalently linked by a single disulfide bond. The p40 subunit, which binds to the receptor chain IL-12Rβ1, is shared with another heterodimeric cytokine, IL-23. However, the two cytokines expert distinct non-redundant biological functions (1). Therapeutic agents targeting both IL-12 and IL-23 cytokines are currently used to treat psoriasis and psoriatic arthritis, and related agents are in clinical testing for a variety of inflammatory disorders (2).

iLite® IL-12 ARCs are a genetically engineered reporter gene cell line (DT-40, ATCC#CRL-2111) responsive to Interleukin-12 by specific and proportional expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.


Products Related to IL-12 Assay Ready Cells

Human IL-12 p40 ELISA Assay Kit
Human IL-12 p70 ELISA Assay Kit
IL-2 Assay Ready Cells

Additional Information

Assay Principle


Interleukin 12 (IL-12) is a 70 kDa cytokine produced principally by macrophages, neutrophils and dendritic cells in response to stimulation by antigen. IL-12 is a T cell-stimulating factor, playing a critical role in the regulation of T-helper 1 cell responses. IL-12 is composed of two subunits, p35 and p40, covalently linked by a single disulfide bond. The p40 subunit, which binds to the receptor chain IL-12Rβ1, is shared with another heterodimeric cytokine, IL-23. However, the two cytokines expert distinct non-redundant biological functions (1). Therapeutic agents targeting both IL-12 and IL-23 cytokines are currently used to treat psoriasis and psoriatic arthritis, and related agents are in clinical testing for a variety of inflammatory disorders (2).
iLite® IL-12 Assay Ready Cells are a genetically engineered reporter gene cell line (DT-40, ATCC#CRL-2111) responsive to Interleukin-12 by specific and proportional expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.

Assay Quick Guide


Click here for the Assay Summary: iLite IL-12 Drug Activity Protocol

Why Choose the iLite® IL-12 Assay Ready Cells?


  • iLite technology employs the cells’ inherent signaling pathways – a true model of cellular events
  • Assay Ready Cells – no culturing required!
  • Highly reproducible between batches
  • Normalization gene allows for consistent results
  • iLite® IL-12 Assay Ready Cells are a genetically engineered reporter gene cell line (DT-40, ATCC#CRL-2111) responsive to Interleukin-12 by specific and proportional expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.

Frequently Asked Questions


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References