iLite RANKL Assay Ready Cells

iLite IL-12 Assay Ready Cells

$1,480.00

The iLite® IL-12 Assay Ready Cells are designed for the specific detection of drug potency in serum/plasma as well as neutralizing antibodies. They utilize an assay technique called reporter gene assay ( RPG ) to analyze serum/plasma samples. The reporter genes utilized are encoded with a bioluminescent luciferase (Firefly luciferase). Different levels of luminescence detected from each reporter gene cell indicates different levels of expression. The iLite® IL-12 Assay Ready Cells are for research use only and not for use in diagnostic or therapeutic procedures.

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iLite IL-12 Assay Ready Cells

iLite IL-12 Assay Ready Cells Developed and Manufactured by Svar Life Science

The Eagle Biosciences iLite IL-12 Assay Ready Cells are intended for:

  • Testing IL-12 or p40 inhibitor activity
  • Testing NAb response against IL-12

For Research Use Only

Key benefits of iLite Assays

  • Highly specific reporter gene cell lines
  • Very sensitive cell line responses (10 fold inductions)
  • Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
  • Assays within a workday (typically 4-7 hour assays)
  • Normalization gene, which eliminates unwanted matrix effects

Assay Background

Interleukin 12 (IL-12) is a 70 kDa cytokine produced principally by macrophages, neutrophils and dendritic cells in response to stimulation by antigen. IL-12 is a T cell-stimulating factor, playing a critical role in the regulation of T-helper 1 cell responses. IL-12 is composed of two subunits, p35 and p40, covalently linked by a single disulfide bond. The p40 subunit, which binds to the receptor chain IL-12Rβ1, is shared with another heterodimeric cytokine, IL-23. However, the two cytokines expert distinct non-redundant biological functions (1). Therapeutic agents targeting both IL-12 and IL-23 cytokines are currently used to treat psoriasis and psoriatic arthritis, and related agents are in clinical testing for a variety of inflammatory disorders (2).

iLite IL-12 ARCs are a genetically engineered reporter gene cell line (DT-40, ATCC#CRL-2111) responsive to Interleukin-12 by specific and proportional expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.

Products Related to IL-12 Assay Ready Cells

Human IL-12 p40 ELISA Assay Kit
Human IL-12 p70 ELISA Assay Kit
IL-2 Assay Ready Cells

Additional Information

Assay Principle

Interleukin 12 (IL-12) is a 70 kDa cytokine produced principally by macrophages, neutrophils and dendritic cells in response to stimulation by antigen. IL-12 is a T cell-stimulating factor, playing a critical role in the regulation of T-helper 1 cell responses. IL-12 is composed of two subunits, p35 and p40, covalently linked by a single disulfide bond. The p40 subunit, which binds to the receptor chain IL-12Rβ1, is shared with another heterodimeric cytokine, IL-23. However, the two cytokines expert distinct non-redundant biological functions (1). Therapeutic agents targeting both IL-12 and IL-23 cytokines are currently used to treat psoriasis and psoriatic arthritis, and related agents are in clinical testing for a variety of inflammatory disorders (2).
iLite® IL-12 Assay Ready Cells are a genetically engineered reporter gene cell line (DT-40, ATCC#CRL-2111) responsive to Interleukin-12 by specific and proportional expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.

Assay Quick Guide

Click here for the Assay Summary: iLite IL-12 Drug Activity Protocol

Why Choose the iLite® IL-12 Assay Ready Cells?

  • iLite technology employs the cells’ inherent signaling pathways – a true model of cellular events
  • Assay Ready Cells – no culturing required!
  • Highly reproducible between batches
  • Normalization gene allows for consistent results
  • iLite® IL-12 Assay Ready Cells are a genetically engineered reporter gene cell line (DT-40, ATCC#CRL-2111) responsive to Interleukin-12 by specific and proportional expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.

Discover the Benefits of iLite

 

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.