iLite IL-6 Assay Ready Cells

$1,190.00

The iLite IL-6 Assay Ready Cells can be used for the quantification of IL-6 activity, IL-6 receptor inhibiting activity or detection of neutralizing antibody response against such inhibitors test samples, including human serum. The Eagle Biosciences iLite IL-6 Assay Ready Cells are for research use only.

SKU: BM4061 Categories: ,

iLite IL-6 Assay Ready Cells

iLite IL-6 Assay Ready Cells Developed and Manufactured by Svar Life Science

The IL-6 Assay Ready Cells are intended for:

    • Quantification of functional IL-6
    • Quantification of IL-6 receptor inhibitor
    • Determination of neutralizing antibodies against IL-6 receptor inhibitor

Content: >250 µL of Assay Ready Cells suspended in cryoprotective medium from Amsbio
Receipt and Storage: Upon receipt confirm that adequate dry-ice is present, and the cells are frozen. Immediately transfer to -80°C storage. Cells should be stored at -80°C (do not store at any other temperature) and are stable as supplied until the expiry date shown. Cells should be used within 30 min of thawing and should be diluted immediately after thawing.
For Research Use Only


Key benefits of iLite® Assays

  • Highly specific reporter gene cell lines
  • Very sensitive cell line responses (>10 fold inductions)
  • Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
  • Assays within a workday (typically 4-7 hour assays)
  • Normalization gene, which eliminates unwanted matrix effects

Product Description

iLite® IL-6 Assay Ready Cells are based on the human embryonic kidney cell line, HEK293 (ATCC# CRL-1573), and have been genetically engineered and optimized to be responsive to human IL-6, resulting in a proportional expression of Firefly Luciferase. Normalization of cell counts, and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.


Products Related to iLite IL-6 Assay Ready Cells

Human IL-6 High Sensitivity ELISA Assay Kit
Human IL-6 ELISA Assay Kit
iLite IL-23 Assay Ready Cells

Additional Information

Background


Interleukin-6 (IL-6) is member of the cytokine family. It is found relevant to any inflammatory diseases and many cancers and can act as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In classic signaling, IL-6 stimulates target cells via a membrane bound interleukin-6 receptor (IL-6R), which upon ligand binding associates with the signaling receptor protein gp130. The anti-inflammatory activities of IL-6 are transmitted by this signaling pathway whereas proinflammatory responses of IL-6 are mediated by trans-signaling (cells expressing only gp130 respond to a complex of IL-6 bound to a soluble form of the IL-6R). This is important since therapeutic inhibition of IL-6 by the neutralizing anti-IL-6R monoclonal antibody has been focused on the treatment of inflammatory diseases (1).

Manual

Product Manual


Frequently Asked Questions