iLite IL-23 Assay Ready Cells

$1,240.00

The iLite IL-23 Assay Ready Cells are designed for the specific detection of drug potency in serum/plasma as well as neutralizing antibodies. They utilize an assay technique called reporter gene assay ( RPG ) to analyze serum/plasma samples. The reporter genes utilized are encoded with a bioluminescent luciferase (Firefly luciferase). Different levels of luminescence detected from each reporter gene cell indicates levels of expression. The iLite IL-23 Assay Ready Cells are for research use only and not for use in therapeutic procedures.

iLite IL-23 Assay Ready Cells

iLite IL-23 Assay Ready Cells Developed and Manufactured by Svar Life Science

The Eagle Biosciences iLite® IL-23 Assay Ready Cells are intended for :

  • Testing IL-23 or p40 inhibitor activity
  • Testing NAb against IL-23 activity

For Research Use Only

Key benefits of iLite® Assays


  • Highly specific reporter gene cell lines
  • Very sensitive cell line responses (>10 fold inductions)
  • Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
  • Assays within a workday (typically 4-7 hour assays)
  • Normalization gene, which eliminates unwanted matrix effects

Assay Principle

Interleukin 23 (IL-23) is a heterodimeric pro-inflammatory cytokine that shares traits with IL-12. Both cytokines contain the p40 subunit which binds to the receptor chain IL-12Rβ1. However, the two cytokines exert distinct non-redundant biological functions. IL-23 has been implicated as an inflammation mediator in several autoimmune diseases, and has also been found to promote tumor growth. Therapeutic agents targeting both IL-12 and IL-23 cytokines are currently used to treat psoriasis and psoriatic arthritis, and related agents are in clinical testing for a variety of inflammatory disorders.


Receipt and Storage

Upon receipt confirm that adequate dry-ice is present, and the cells are frozen. Immediately transfer to -80°C storage. Cells should be stored at -80°C (do not store at any other temperature) and are stable as supplied until the expiry date shown. Cells should be used within 30 min of thawing and should be diluted immediately after thawing.

Products related to iLite IL-23 Assay Ready Cells

iLite IL-2 Assay Ready Cells
iLite IL-12 Assay Ready Cells
iLite IL-6 Assay Ready Cells

Additional Information

Background


Interleukin 23 (IL-23) is a heterodimeric pro-inflammatory cytokine that shares traits with IL-12. Both cytokines contain the p40 subunit which binds to the receptor chain IL-12Rβ1. However, the two cytokines exert distinct non-redundant biological functions. IL-23 has been implicated as an inflammation mediator in several autoimmune diseases, and has also been found to promote tumor growth. Therapeutic agents targeting both IL-12 and IL-23 cytokines are currently used to treat psoriasis and psoriatic arthritis, and related agents are in clinical testing for a variety of inflammatory disorders.

Assay Quick Guide


Click here for the Assay Summary: IL-23 Activity Protocol

Why Choose the iLite® IL-23 Assay Ready Cells?


  • iLite technology employs the cells’ inherent signaling pathways – a true model of cellular events
  • Assay Ready Cells – no culturing required!
  • No protein tagging or labeling required
  • Short assay times
  • IL-23 inhibitor activity: 30min + 5h
  • Neutralizing antibodies: 30min + 30 min + 5h
  • Highly reproducible between batches
  • Normalization gene allows for consistent results
  • Adjusts for serum matrix effects and differences in cell number
  • Highly specific for IL-23, no cross-talk with IL-12

Frequently Asked Questions


Manual

Product Manual


Publications

References