iLite® IL-23 Assay Ready Cells

$1,240.00

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iLite® IL-23 Assay Ready Cells :

  • The iLite® IL-23 Assay Ready Cells can be used for the quantification of IL-23 or p40 inhibitor activity, or the neutralizing antibody response against such inhibitors in human serum or buffer.
  • Content:  >250 µL of Assay Ready Cells suspended in RPMI 1640 medium with 20% heat inactivated fetal bovine serum (FBS) and 10% dimethyl sulfoxide (DMSO).
  • Storage:  -80°C, Cells should be used within 30 min of thawing.
  • Application notes for the following assays are available:

Product Developed and Manufactured by EuroDiagnostica
For Research Use Only

Background

Interleukin 23 (IL-23) is a heterodimeric pro-inflammatory cytokine that shares traits with IL-12. Both cytokines contain the p40 subunit which binds to the receptor chain IL-12Rβ1. However, the two cytokines exert distinct non-redundant biological functions. IL-23 has been implicated as an inflammation mediator in several autoimmune diseases, and has also been found to promote tumor growth. Therapeutic agents targeting both IL-12 and IL-23 cytokines are currently used to treat psoriasis and psoriatic arthritis, and related agents are in clinical testing for a variety of inflammatory disorders.

 

Why Choose the iLite® IL-23 Assay Ready Cells?

  • iLite technology employs the cells’ inherent signaling pathways – a true model of cellular events
  • Assay Ready Cells – no culturing required!
  • No protein tagging or labeling required
  • Short assay times
    • IL-23 inhibitor activity: 30min + 5h
    • Neutralizing antibodies: 30min + 30 min + 5h
  • Highly reproducible between batches
  • Normalization gene allows for consistent results
  • Adjusts for serum matrix effects and differences in cell number
  • Highly specific for IL-23, no cross-talk with IL-12

 

The Eagle Bio iLite Assay Ready Cells are designed for the specific detection of drug potency in serum/plasma as well as neutralizing antibodies. They utilize an assay technique called reporter gene assay ( RPG ) to analyze serum/plasma samples. The reporter genes utilized are encoded with a bioluminescent luciferase (Firefly luciferase). Different levels of luminescence detected from each reporter gene cell indicates levels of expression.

Key benefits of iLite® Assays:

  • Highly specific reporter gene cell lines
  • Very sensitive cell line responses (>10 fold inductions)
  • Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
  • Assays within a workday (typically 4-7 hour assays)
  • Normalization gene, which eliminates unwanted matrix effects