The Human ERBB3/Her3 ELISA Kit is intended for the quantitation of Human ERBB3 concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human ERBB3/Her3 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: HR331-K01 Categories: , ,


The Human ERBB3 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 156pg/ml-10000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma(heparin, EDTA)
Sample Size: 100 µl
Alternative Names: Her3, Receptor tyrosine-protein kinase erbB-3, human epidermal growth factor receptor 3

Assay Principle

The Human ERBB3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human ERBB3 with a 96-well strip plate that is pre-coated with antibody specific for ERBB3. The detection antibody is a biotinylated antibody specific for ERBB3. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human ERBB3 with immunogen: NSO, S20-T643. The kit is analytically validated with ready to use reagents. To measure Human ERBB3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human ERBB3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human ERBB3 in the sample.

Related Products

Human ErbB-2 ELISA Kit
Human Serpin A7 ELISA Kit
Human Annexin A1 ELISA Kit

Additional Information

Assay Background

Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene. ErbB3 has been shown to bind the ligands heregulin and NRG-2. Ligand binding causes a change in conformation that allows for dimerization, phosphorylation, and activation of signal transduction. ErbB3 can heterodimerize with any of the other three ErbB family members. The theoretical ErbB3 homodimer would be non-functional because the kinase-impaired protein requires transphosporylation by its binding partner to be active. Unlike the other ErbB receptor tyrosine kinase family members which are activated through autophosphorylation upon ligand binding, ErbB3 is found to be kinase impaired, having only 1/1000th the autophosphorylation activity of EGFR and no ability to phosphorylate other proteins. Therefore, ErbB3 must act as anallosteric activator.

Typical Standard Curve



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