Human Annexin A1 ELISA Kit


The Eagle Biosciences Human Annexin A1 ELISA Kit is intended for the quantitation of Human ANXA1 concentrations in cell culture supernates, serum and plasma (heparin, EDTA, citrate). The Eagle Biosciences Human Annexin A1 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: ANX31-K01 Categories: , ,

Human Annexin A1 ELISA Kit

The Human Annexin A1 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <100pg/ml
Standard Range: 156pg/ml-10000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, Serum and Plasma (heparin, EDTA, citrate).
Sample Size: 100 µl
Alternative Names: ANXA1, Lipocortin I

Assay Principle

The Human ANXA1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human ANXA1 with a 96-well strip plate that is pre-coated with antibody specific for ANXA1. The detection antibody is a biotinylated antibody specific for ANXA1. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human ANXA1 with immunogen: Expression system for standard: NSO, Immunogen sequence: A2-N346. The kit is analytically validated with ready to use reagents. To measure Human ANXA1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human ANXA1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human ANXA1 in the sample.

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Additional Information

Assay Background

ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.

Typical Standard Curve

Human Annexin A1 ELISA Kit

Package Inserts

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