Human ErbB-2 ELISA Kit

$520.00

The Human ErbB-2 ELISA Kit is intended for the quantitation of ERBB2 concentrations in cell culture supernates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human ErbB-2 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: RBB31-K01 Categories: , ,

Human ErbB-2 ELISA Kit

The Human ErbB-2 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 15.6pg/ml-1000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA)
Sample Size: 100 µl
Alternative Names: HER2/neu, Human Epidermal growth factor Receptor 2, CD340, cluster of differentiation 340, p185


Assay Principle

The Human ERBB2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human ERBB2 with a 96-well strip plate that is pre-coated with antibody specific for ERBB2. The detection antibody is a biotinylated antibody specific for ERBB2. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human ERBB2 with immunogen: Expression system for standard: NSO; Immunogen sequence: T23-T652. The kit is analytically validated with ready to use reagents. To measure Human ERBB2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human ERBB2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human ERBB2 in the sample.


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Additional Information

Assay Background


HER2/neu(also known as ErbB-2) stands for “Human Epidermal growth factor Receptor 2” and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2/neu has also been designated as CD340(cluster of differentiation 340) and p185. It is encoded by the ERBB2 gene.HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. It is encoded within the genome by HER2/neu, a known proto-oncogene. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. However, ErbB receptors dimerise on ligand binding, and HER2 is the preferential dimerisation partner of other members of the ErbB family.1 The HER2 gene is a proto-oncogene located at the long arm of human chromosome 17(17q21-q22)2.

Typical Standard Curve


Human ErbB-2 ELISA Kit

Documents

Product Manual