Human Complement C5a ELISA Kit

$570.00

The Eagle Biosciences Human Complement C5a ELISA Kit is intended for the quantitation of Human C5 concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human complement C5a ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: C5A31-K01 Categories: , ,

Human Complement C5a ELISA Kit

The Human Complement C5a ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 31.2pg/ml-2000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma(heparin, EDTA)
Sample Size: 100 µl


Assay Background

C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.


Related Products

Human Complement H/CFH ELISA Kit
Complement TCC ELISA Kit
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Additional Information

Assay Principle


The Human C5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human C5 with a 96-well strip plate that is pre-coated with antibody specific for C5. The detection antibody is a biotinylated antibody specific for C5. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human C5 with immunogen: Expression system for standard: E.coli; Immunogen sequence: T678-R751. The kit is analytically validated with ready to use reagents. To measure Human C5, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human C5 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human C5 in the sample.

Typical Standard Curve


Documents


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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