Mouse VEGFR2/KDR ELISA Kit

$570.00

The Mouse VEGFR2/KDR ELISA Kit is intended for the quantitation of Mouse KDR concentrations in cell culture supernates and serum. The Eagle Biosciences Mouse VEGFR2/KDR ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: KDR11-K01 Categories: , ,

Mouse VEGFR2/KDR ELISA Kit

The Mouse VEGFR2/KDR ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <2pg/ml
Standard Range: 62.5pg/ml-4000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates and serum
Sample Size: 100 µl
Storage Instructions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freezethaw cycles (Shipped with wet ice.)
Alternative Names: Vascular endothelial growth factor receptor 2, kinase insert domain receptor


Assay Background

Vascular endothelial growth factor receptor 2(VEGFR-2) is a VEGF receptor. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It is mapped to 4q12. This gene encodes one of the two receptors of the VEGF. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells. In addition to those, VEGFR-2 is also a positive functional marker defining stem cells and distinguishing them from progenitors.


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Additional Information

Assay Principle


The Mouse KDR Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse KDR with a 96-well strip plate that is pre-coated with antibody specific for KDR. The detection antibody is a biotinylated antibody specific for KDR. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse KDR with immunogen: Expression system for standard: NSO; Immunogen sequence: A20-E762. The kit is analytically validated with ready to use reagents. To measure Mouse KDR, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse KDR in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse KDR in the sample.

Typical Standard Curve


Mouse VEGFR2/KDR ELISA Kit

Documents

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Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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