P1NP ELISA Assay Kit


The Procollagen Type 1 N-Terminal Propeptide (P1NP) ELISA Assay Kit is designed for quantification of human P1NP in serum and plasma samples. The Eagle Biosciences P1NP ELISA Assay Kit is for research use only and not to be used for diagnostic purposes.

SKU: PNP31-K01 Categories: , ,

P1NP ELISA Assay Kit

The P1NP ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 62.5pg/mL
Standard Range: 62.5pg/mL-4000pg/mL
Incubation Time: 2 hours 35 minutes
Sample Type: Serum and plasma
Sample Size: 100 µl
Alternative Names: Procollagen Type 1 N-Terminal Propeptide, ProCollA1

Assay Principle

The Procollagen Type 1 N-Terminal Propeptide (P1NP) ELISA Assay Kit is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human P1NP. Standards and samples are pipetted into the wells and any human P1NP present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human P1NP is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human P1NP bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human P1NP, the unknown sample concentration can be interpolated from a reference curve included in each assay.

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Additional Information

Assay Background

In bone, collagen type I is synthesized by osteoblasts in the form of procollagen. This precursor contains a short signal sequence and terminal extension peptides: amino-terminal propeptide (PINP) and carboxy-terminal propeptide. These propeptide extensions are removed by specific proteinases before the collagen molecules form. Both propeptides can be found in the circulation and their concentration reflects the synthesis rate of collagen type I.

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