P1NP ELISA Assay Kit
The P1NP ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 62.5pg/mL
Standard Range: 62.5pg/mL-4000pg/mL
Incubation Time: 2 hours 35 minutes
Sample Type: Serum and plasma
Sample Size: 100 µl
Alternative Names: Procollagen Type 1 N-Terminal Propeptide, ProCollA1
Assay Principle
The Procollagen Type 1 N-Terminal Propeptide (P1NP) ELISA Assay Kit is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human P1NP. Standards and samples are pipetted into the wells and any human P1NP present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human P1NP is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human P1NP bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human P1NP, the unknown sample concentration can be interpolated from a reference curve included in each assay.
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