Eagle Biosciences, Author at Eagle Biosciences

Publication Spotlight

NT-proCNP ELISA Used in New Study

The Eagle Biosciences NT-proCNP ELISA Assay Kit (manufactured by Biomedica) was used in a recent study published out of the University of Otago in Christchurch, New Zealand. This kit is used for the quantitative determination of NT-proCNP in human serum and plasma (EDTA, citrate, heparin). CNP, C-type natriuretic peptide, along with other natriuretic peptides, plays a role in regulatory mechanisms including blood pressure and body fluid homeostasis. It also serves as a biomarker.

Plasma C-Type Natriuretic Peptide: Emerging Applications in Disorders of Skeletal Growth

Abstract

Although studies in experimental animals show that blood levels of C-type natriuretic peptide (CNP) and its bioinactive aminoterminal propeptide (NTproCNP) are potential biomarkers of long bone growth, a lack of suitable assays and appropriate reference ranges has limited the application of CNP measurements in clinical practice. Plasma concentrations of the processed product of proCNP, NTproCNP – and to a lesser extent CNP itself – correlate with concurrent height velocity throughout all phases of normal skeletal growth, as well as during interventions known to affect skeletal growth in children. Since a change in levels precedes a measurable change in height velocity during interventions, measuring NTproCNP may have predictive value in clinical practice. Findings from a variety of genetic disorders affecting CNP signaling suggest that plasma concentrations of both peptides may be helpful in diagnosis, provided factors such as concurrent height velocity, feedback regulation of CNP, and differential changes in peptide clearance are considered when interpreting values. An improved understanding of factors affecting plasma levels, and the availability of commercial kits enabling accurate measurement using small volumes of plasma, can be expected to facilitate potential applications in growth disorders including genetic causes affecting the CNP signaling pathway.

Espiner, E.; Prickett, T.; Olney, Robert; (2019). Plasma C-Type Natriuretic Peptide: Emerging Applications in Disorders of Skeletal Growth. Horm. Res. Paediatr., 90:345–357. DOI: 10.1159/000496544

Technical Spotlight

Technical Spotlight: DotDiver

by Eagle Biosciences

DotDiver2.0 is a fully automatic processor for Dot/LINE immunoassays manufactured by German biotech Generic Assays.

DotDiver2.0 processes and evaluates assay kits with minimal user intervention. It can perform up to 24 tests at one time. It features bar code identification for test strips and cartridges. DotDiver provides digitized results, and it is LIMS ready or can be set up to print on paper. All you have to do is load the tests and press start, no need to monitor while it’s running. The system requires minimal maintenance and takes up little space.

DotDiver2.0 assays are available for:

Celiac disease / Crohn‘s disease
Pernicious anemia
Neuropathies
Anti-phospholipid syndrome
Systemic lupus erythematosus
Vasculitis
Nephritis
Myositis
Scleroderma
Autoimmune hepatitis

For more information, see the DotDiver 2.0 Immunoassay Processor product page.

Trade Show Spotlight

Come see us at AMLI!

by Eagle Biosciences

Eagle Biosciences will be at The Association of Medical Laboratory Immunologists (AMLI) Annual Scientific Meeting in Cleveland, OH, August 16-19. The conference is being held at the Renaissance Cleveland.

We will be at booth #8, and we would love to meet you! Stop by and say hi, or reach out if you’d like to set up a meeting.

Eagle News

Comparison between the AroCell TK 210 ELISA and TK1 enzyme activity assays

by Eagle Biosciences

Comparison between the AroCell TK 210 ELISA and TK1 enzyme activity assays

AroCell recently completed a comparison between their 210 ELISA with the ABCAM TK1 ELISA and the Gold-Standard Tritiated TK1 Activity Assay to confirm the diagnostic accuracy of each kit. The results found that the AroCell kit is an accurate assay for the determination of thymidine kinase 1.

Read the report here.

Eagle Biosciences offers the AroCell TK 210 ELISA as part of our product catalog:
TK 210 (Thymidine Kinase 1 (TK1)) ELISA Assay Kit

New Product Series

Product Category Spotlight: easYmers

by Eagle Biosciences

Through our collaboration with ImmunAware, we are excited to offer a new range of products: easYmer MHC Tetramers. This product allows laboratories to easily generate monomers using their choice of peptide. The resulting monomers can also be easily tetramerized using fluorophore conjugated streptavidin. These tetramers can then be used to stain cognate T cells for flow cytometry. easYmers allow end users to rapidly produce large quantities of pMHC monomers with a single epitope, to produce and screen pMHC monomers with many different peptide epitope candiates in parallel, and to validate peptide HLA bonding.

Click here to view our available easYmer MHC tetramer kits.

If you have questions about easYmers or if you’re looking for a specific kit, contact us and we’ll be happy to assist.

Supplier Agreement Announcements

Eagle Welcomes ImmunAware!

by Eagle Biosciences

Eagle Biosciences, Inc. is excited to announce that we have partnered with ImmunAware, based in Denmark, to offer their line of easYmer MHC tetramer products in the U.S. and Canada.

About ImmunAware

ImmunAware originated from the Experimental Immunology lab at the University of Copenhagen. They specialize in major histocompatibility complex (MHC) molecules. They offer a broad range of peptide-MHC class I and II complexes as either biotinylated monomers, or as tetramers. Their easYmer product line allows laboratories to generate their own peptide-MHC monomers and tetramers.

About easYmers

easYmers contain an active formulation of peptide receptive HLA-I molecules that facilitates monomer generation. The resulting monomers are easily tetramerized with fluorophore conjugated streptavidin for use in flow cytometry for T-cell analysis. easYmer reagents can also be used to evaluate specific peptide-HLA I binding.

Technical Spotlight

Technical Spotlight: Metal Enhanced Fluorescence Assays

by Eagle Biosciences

What is Metal Enhanced Fluorescence?

Metal Enhanced Fluorescence (MEF) is based on the reaction of excitation light with electrons of metal nano-structures to generate strong electromagnetic fields, also known as LSPs (localized surfaced plasmons). When LSPs are bound to the surface of the metal nano-structure, it creates a plasmonic structure, which can enhance the signal of the phosphores more than 100 times.

About our FluoBolt™ MEF Assays

Eagle Biosciences proudly offers a line of Metal Enhanced Fluorescence Immunoassays from our partner, Fianostics. These products from the FluoBolt™ line are highly sensitive, single-step assays that require no enzyme substrates. FluoBolt™ MEFs are completely compatible with the 96-well ELISA format and provide a stable signal over time.

We currently offer the following FluoBolt™ MEF Immunoassays:

With many more to come!

Publication Spotlight

Norepinephrine ELISA Used in Recent Study

by Eagle Biosciences

The Eagle Biosciences Noradrenaline (Norepinephrine) Sensitive ELISA was used in a recent study. This highly sensitive assay kit is part of our veterinary and neurobiology product lines. It is intended for the detection of noradrenaline (norepinephrine) in biological samples including serum, plasma, tissue, and cell culture samples of mice and rats.

Renal Denervation and CD161a Immune Ablation Prevent Cholinergic Hypertension and Renal Sodium Retention

Abstract

Cholinergic receptor activation leads to premature development of hypertension and infiltration of pro-inflammatory CD161a+/CD68+ M1 macrophages into the renal medulla. Renal inflammation is implicated in renal sodium retention and the development of hypertension. Renal denervation is known to decrease renal inflammation. To determine the role of CD161a+/CD68+ macrophages and renal sympathetic nerves in cholinergic-hypertension & renal sodium retention. Bilateral renal denervation (RND) and immune ablation of CD161a+ immune cells was performed in young prehypertensive SHR followed by infusion of either saline or nicotine (15mg/kg/day) for two weeks. Immune ablation was conducted by injection of unconjugated azide-free antibody targeting rat CD161a. Blood pressure was monitored by tail cuff plethysmography. Tissues were harvested at the end of infusion. Nicotine induced premature hypertension, renal expression of the sodium-potassium-chloride co-transporter (NKCC2), increases in renal sodium retention, and infiltration of CD161a+/CD68+ macrophages into the renal medulla of animals. All of these effects were abrogated or prevented by RND and ablation of CD161a+ immune cells. Cholinergic activation of CD161a+ positive immune cells leads to the premature development of hypertension in SHR, at least partly, through increased renal expression of NKCC2 and renal sodium retention. Effects on chemotaxis of CD161a+ macrophages to the renal medulla, decreased renal expression of NKCC2, and renal sodium retention appear to play a part in the prevention of cholinergic hypertension as a result of RND. The CD161a+ immune cells are necessary and essential for this pro-hypertensive nicotine-mediated inflammatory response. Cholinergic receptor activation leads to premature development of hypertension and infiltration of pro-inflammatory CD161a+/CD68+ M1 macrophages into the renal medulla. Renal inflammation is implicated in renal sodium retention and the development of hypertension. Renal denervation is known to decrease renal inflammation. To determine the role of CD161a+/CD68+ macrophages and renal sympathetic nerves in cholinergic-hypertension and renal sodium retention. Bilateral renal denervation (RND) and immune ablation of CD161a+ immune cells was performed in young prehypertensive SHR followed by infusion of either saline or nicotine (15mg/kg/day) for two weeks. Immune ablation was conducted by injection of unconjugated azide-free antibody targeting rat CD161a. Blood pressure was monitored by tail cuff plethysmography. Tissues were harvested at the end of infusion. Nicotine induced premature hypertension, renal expression of the sodium-potassium-chloride co-transporter (NKCC2), increases in renal sodium retention, and infiltration of CD161a+/CD68+ macrophages into the renal medulla of animals. All of these effects were abrogated or prevented by RND and ablation of CD161a+ immune cells. Cholinergic activation of CD161a+ positive immune cells leads to the premature development of hypertension in SHR, at least partly, through increased renal expression of NKCC2 and renal sodium retention. Effects on chemotaxis of CD161a+ macrophages to the renal medulla, decreased renal expression of NKCC2, and renal sodium retention appear to play a part in the prevention of cholinergic hypertension as a result of RND. The CD161a+ immune cells are necessary and essential for this pro-hypertensive nicotine-mediated inflammatory response.

Raikwar, NS;Braverman, C;Snyder, PM;Fenton, RA;Meyerholz, DK;Abboud, FM;Harwani, SC; (2019). Renal Denervation and CD161a Immune Ablation Prevent Cholinergic Hypertension and Renal Sodium Retention. Am. J. Physiol. Heart Circ. Physiol.. doi:10.1152/ajpheart.00234.2019.

Biomarker Spotlight

Biomarker Spotlight: Noggin

by Eagle Biosciences

Noggin is a secreted homodimeric glycoprotein that is involved in the development of many body tissues, including nerve tissue, muscles, and bones. Noggin is crucial during embryogenesis and is involved in the regulation of several developmental processes, including neural tube formation, cardiomycyte growth, skeletal development, and joint formation. Noggin is highly conserved in vertebrates, and is found in certain areas of the central nervous system, lungs, skeletal muscles, and skin of adults.

Why is it important?

Research has shown that noggin plays a role in learning, cognition, bone development, and neural tube function, as well as nervous system, somite and skeletal development. A lack of noggin during embryo development can lead to stunted bone growth, missing skeletal elements, or failure to develop articulating joints. Mutations of the noggin gene can cause joint fusions, multiple synostosis, proximal symphalangism and other deformities or syndromes.

In adult patients, increased levels of noggin in plasma have been linked to obesity.

NOGGIN has also been linked to:
Bone Tumors
Ankylosing Spondylitis
Non Alcoholic Fatty Liver Disease (NAFLD)
Pulmonary Arterial Hypertension (PAH)

Eagle Biosciences offers a highly sensitive Metal Enhanced Direct Sandwich Fluorescence Immunoassay to test for Noggin.
FluoBolt-Noggin Fluorescence Immunoassay

Uncategorized

GFAP: Novel Test for a Newly Identified Meningoencephalomyelitis

by Eagle Biosciences

GFAP: Novel Test for a Newly Identified Meningoencephalomyelitis

Mayo Clinic

Meningoencephalomyelitis is swelling of the brain or spinal cord caused by an autoimmune reaction. Symptoms include headaches, progressive weakness, confusion and blurred vision. This condition can be treated with steroid therapy, but it is often misdiagnosed as meningitis, encephalitis, myelitis, or a variety of other conditions.

Researchers have developed a unique test to detect a novel form of the disease known as autoimmune glilial fibrillary acidic protein (GFAP) astrocytopathy. This test would be part of encephalitis and myelopathy evaluations. The recommended test involves a test of the patient’s spinal fluid, as well as a serum test.

Toman, Barbara J. “GFAP: Novel Test for a Newly Identified Meningoencephalomyelitis.” Mayo Clinic Laboratories, https://news.mayocliniclabs.com/2019/06/28/gfap/.

Eagle Biosciences offers the most sensitive assays available worldwide in simple-to-use ELISA and Chemiluminescence formats for detecting GFAP: