Noradrenaline (Norepinephrine) Sensitive ELISA
Noradrenaline Sensitive ELISA manufactured in Germany as SKU EA633/96 by DLD Diagnostika
Size: 1×96 wells
Sensitivity: 1.0 pg/mL
Dynamic Range: 0.05 – 25 ng/ml
Incubation Time: Overnight
Sample Type: Biological Samples
Sample Size: 20 µL
Alternative Names: Norepinephrine Sensitive ELISA, Noradrenaline High Sensitive ELISA, Norepinephrine High Sensitive ELISA
For Research Use Only
Conversion into pmol/l: Noradrenaline: 1 pg/ml = 5.91 pmol/l
Assay Introduction for Noradrenaline Sensitive ELISA
The Noradrenaline (Norepinephrine) High Sensitive ELISA provides materials for the quantitative measurement of noradrenaline in low concentrated samples and for small sample volumes. Noradrenaline is extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline and then converted enzymatically into N-acylnormetanephrine.
The Noradrenaline (Norepinephrine) High Sensitive ELISA is competitive and uses the microtiter plate format. In the Norepinephrine assay, the noradrenaline is bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.