L-Lysine ELISA Assay Kit

$1,185.00

The L-Lysine ELISA Assay Kit is a competitive ELISA kit for the quantitative measurement of L-Lysine (Lys) in plasma samples. The L-Lysine ELISA Assay Kit is For research use only.

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L-Lysine ELISA Assay Kit

L-Lysine ELISA Assay Kit Developed and Manufactured in France by ImmuSmol

Size: 1×96 wells
Sensitivity: 2.2 µg/mL
Standard Range: 7.42 – 118.72 µg/mL
Incubation Time: Overnight
Sample Type: Plasma
Sample Size: 20µL
For Research Use Only

Controls Included

Assay Principle

The L-Lysine ELISA Assay Kit (Enzyme Immunoassay) is for the quantitative determination of L-Lysine (Lys) in plasma samples. After extraction and derivatization L-Lysine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of L-Lysine in unknown samples is achieved by comparing their absorbance with a reference curve prepared with known L-Lysine standards.

Storage and Stability

The date is indicated on the L-Lysine ELISA kit labels. Once opened the reagents are stable for 1 month when stored at 2 – 8 °C. Once the L-Lysine resealable pouch has been opened, care should be taken to close it tightly with desiccant again.

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Additional Information

Assay Background

L-Lysine (Lys) is an essential amino acid since it’s not produced in the body and must be supplied through the diet. It’s one of the building blocks for protein, peptide and non-peptide synthesis, and is thus found involved in several physiological and biochemical processes. As a result, Lys deficiency was shown to affect cell cycle, apoptosis, amino acid metabolism, and to even impair antibody responses as well as cell-mediated immune cell functions. In addition, and like other amino acids, Lys contributes to epigenetic and protein regulations and immunosuppression ; its catabolism activation generates acetyl-CoA which, for instance, promotes Wnt signaling and can support tumor growth.

Furthermore, imbalance in circulating Lys levels can be observed in many conditions such as i) inherited diseases on enzymes involved in Lys catabolism (e.g. glutaric aciduria type I or antiquitin deficiency), ii) diabetes-dependent cardiovascular disease, as well as iii) human immunodeficiency viruses-1 infection.

Typical Standard Curve

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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