ADMA ELISA Assay Kit
ADMA ELISA Assay Kit is developed and manufactured in Germany by DLD Diagnostika EA212/96 (Previously EA201/96)
For Research Use Only
Size: 1×96 wells
Sensitivity: 0.03 µmol/L
Dynamic Range: 0.2 – 3.0 µmol/L
Incubation Time: < 3 hours
Sample Type: Serum, Plasma
Sample Size: 20 µL
Alternative Names: Asymmetric Dimethylarginine
Controls Included
Reference Values (Normal)
0.4 – 0.75 µmol/l (80 – 150 ng/ml)
Each laboratory should establish its own normal ranges
Assay Principle
The competitive ADMA (Asymmetric Dimethylarginine) ELISA uses the microtiter plate format. ADMA is bound to the solid phase of the microtiter plate. ADMA in the samples is acylated and competes with solid phase bound ADMA for a fixed number of rabbit anti-ADMA antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase ADMA is detected by anti-rabbit/peroxidase and the substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase ADMA is inversely proportional to the ADMA concentration of the sample.
Products Related to ADMA ELISA Assay
ADMA Arginine ELISA Assay Kit
Homoarginine ELISA Assay Kit
SDMA ELISA Assay Kit
https://eaglebio.com/product/sdma-elisa-assay-kit/Related News
For a Full Listing of Publications utilizing these ADMA ELISA Assays, click on the link below:
ADMA ELISA Kit Publications
Learn more about ADMA at Eagle Biosciences Biomarker Spotlight page dedicated to ADMA here:
ADMA Biomarker Spotlight
Publications
Citations
Approach. JSM. 47:149-155.
Lee, SJ;Lee, JS;Choi, MH;Lee, SE;Shin, DH;Hong, JM;, (2017). Cilostazol improves endothelial function in acute cerebral ischemia patients: a double-blind placebo controlled trial with flow-mediated dilation technique. BMC Neurol, 17(1), 169-
Badran, M;Abuyassin, B;Golbidi, S;Ayas, N;Laher, I;, (2016). Uncoupling of Vascular Nitric Oxide Synthase Caused by Intermittent Hypoxia. Oxid Med Cell Longev, 20162354870-PubMed ID: 2784066
Baltgalvis, KA;White, K;Li, W;Claypool, MD;Lang, W;Alcantara, R;Singh, BK;Friera, AM;McLaughlin, J;Hansen, D;McCaughey, K;Nguyen, H;Smith, IJ;Godinez, G;Shaw, SJ;Goff, D;Singh, R;Markovtsov, V;Sun, TQ;Jenkins, Y;Uy, G;Li, Y;Pan, A;Gururaja, T;Lau, D;Park, Exercise performance and peripheral vascular insufficiency improve with AMPK activation in high-fat diet-fed mice, Am. J. Physiol. Heart Circ. Physiol., 2014, Volume 36, Issue 8, page H1128-45, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989755/
Schulze F, Wesemann R, Schwedhelm E, Sydow K, Albsmeier J, Cooke JP, Böger RH. Determination of ADMA using a novel ELISA assay.Clin. Chem. Lab. Med. 2004; 42: 1377-1383
Krempl TK, Kähler J, Maas R, Silberhorn L, Meinertz T, Böger RH.Elevation of asymmetric dimethylarginine (ADMA) in patients with unstable angina and recurrent cardiovascular events.Eur. Heart J. 2005; 26: 1846-1851
Schulze F, Maas R, Freese R, Schwedhelm E, Silberhorn L, Böger RH.Determination of a reference value for N,N-dimethyl-L-arginine in 500 subjects.Eur. J. Clin. Invest. 2005; 35 : 622-626
Schnabel R, Blankenberg S, Lubos E, Lackner KJ, Rupprecht HJ, Espinola-Klein C, Jachmann N, Post F, Peetz D, Bickel C, Cambien F, Tiret L, Münzel T. Asymmetric dimethylarginine and the risk of cardiovascular events and death in patients with coronary artery disease: results from the AtheroGene Study.Circ. Res. 2005; 97: e53-59
O`Dwyer MJ, Dempsey F, Crowley V, Kelleher D, McManus R, Ryan T.Septic shock correlates with ADMA levels which may be influenced by a polymorphism in DDAH II: a prospective observational study. Crit. Care 2006; 10: (5): R139 Antoniades C, Tousoulis D, Marinou K, Vasiliadou C, Tentolouris C, Bouras G, Pitsavos C, Stefanidis C. Asymmetrical dimethylarginine regulates endothelial function in methionine-induced but not in chronic homocystinemia in humans: effect of oxidative stress and proinflammatory cytokines Am. J. Clin. Nutr. 2006; 84: 781-788
Wang TZ., Chen WJ., Cheng WC., Lin JW., Chen MF., Lee YT.Relation of improvement in endothelium-dependent flowmediated vasodilation after Rosiglitazone to changes in asymmetric dimethylarginine, endothelin-1, and C-reactive protein in nondiabetic patients with the metabolic syndromeAm. J. Cardiol. 2006; 9: 1057-1062
Wanby P., Nilsson I., Brudin L., Nyhammar I., Gustafsson I., Carlsson M. Increased plasma levels of asymmetric dimethylarginine in patients with carotid stenosis: no evidence for the role of the common FABBP2 A54T gene polymorphism >Acta Neurol. Scand. 2007; 115: 90-96
Konishi H, Sydow K, Cooke JP.Dimethylarginine dimethylaminohydrolase promotes endothelial repair after vascular injury J. Am. Coll. Cardiol. 2007; 49: 1099-1105
Iribarren C, Husson G, Sydow K, Wang BY, Sidney S, Cooke JP. Asymmetric dimethyl-arginine and coronary artery calcification in young adults entering middle age: the CARDIA Study Eur. J. Cardiovasc. Prev. Rehabil. 2007; 14:222-229
Melikian N, Wheatcroft SB, Ogah OS, Murphy C, Chowienczyk PJ, Wierzbicki AS, Sanders TA, Jiang B, DuncanER, Shah AM, Kearney MT.Asymmetric dimethylarginine and reduced nitric oxide bioavailability in young Black African men Hypertension 2007; 49: 873-877
Horowitz JD, Heresztyn T. An overview of plasma concentrations of asymmetric dimethylarginine (ADMA) in health and disease and in clinical studies: Methodological considerations. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2007 Charitidou C, Farmakiotis D, Zournatzi V, Pidonia I, Pegiou T, Karamanis N, Hatzistilianou M, Katsikis I, Panidis D. The administration of estrogens, combined with anti-androgens, has beneficial effects on the hormonal features and asymmetric dimethyl-arginine levels, in women with the polycystic ovarysyndrome Atherosclerosis 2007; in press.