L-Serine ELISA Assay Kit

$880.00

The L-Serine ELISA Assay kit  is an enzyme immunoassay for the quantitative determination of L-Serine (Ser) in plasma samples or culture supernatant. The L-Serine ELISA Assay kit is for Research Use Only.

L-Serine ELISA Assay Kit

L-Serine ELISA Assay Kit Developed and Manufactured in France by ImmuSmol

Size: 1×96 wells
Sensitivity: 0.25 µg/ml
Standard Range: 1.68 – 65.68 µg/ml
Incubation Time: Overnight
Sample Type: Plasma and Cell Supernatant
Sample Size: 50µL
For Research Use Only

Controls Included


Background

The L-Serine ELISA Assay kit is an enzyme immunoassay for the quantitative determination of L-Serine (Ser) in plasma samples or culture supernatant. After extraction and derivatization L-Serine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.

Specificity: No significant cross-reactivity was observed with Glycine, L-Threonine, L-Cysteine, D-Serine, or O-Phospho-L-Serine with the The L-Serine ELISA Assay kit.


Sample Collection and Storage

EDTA Plasma

  • Whole blood should be collected into centrifuge tubes containing EDTA as anti-coagulant (Monovette™ or Vacuette™ for plasma) and centrifuged according to manufacturer’s instructions at room temperature immediately after collection.
  • Haemolytic and especially lipemic samples should not be used for the assay.
  • Storage: up to 48 hours at 2 – 8 °C, for longer period (up to 6 month) at -20 °C.
  • Repeated freezing and thawing should be avoided with the L-Serine ELISA Assay kit .

Culture Media

  • Culture media should be collected into centrifuge tubes and centrifuged in order to remove pellet cells. Supernatant could be processed immediately or frozen undiluted at -80°C for longer period. If necessary and for a better accuracy, pre-dilute the sample in PBS to have an expected concentration around the standard D (10,51µg/mL – 100µmol/L).
  • Repeated freezing and thawing should be avoided with the L-Serine ELISA Assay kit.

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Additional Information

Assay Procedure


Allow all reagents and samples to reach room temperature and mix thoroughly by gentle inversion before use. Duplicate determinations are recommended. To do that, perform duplicates after derivatization (1 derivatization well = 2 competition wells). It is recommended to number the strips of the microwell plate before usage to avoid any mix-up. The binding of the antisera and of the enzyme conjugate and the activity of the enzyme are temperature dependent, and the absorbance values may vary if a thermostat is not used. The higher the temperature, the higher the extinction values will be. Corresponding variations also apply to the incubation times. The optimal temperature during the Enzyme Immunoassay is between 20 – 25 °C.

Typical Standard Curve


 

Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations