Folic Acid Microtiter Plate Assay
Folic Acid Microtiter Plate Assay manufactured in Germany by Immundiagnostik
Size: 1×96 wells
Sensitivity: 0.04 µg/L
Dynamic Range: 0.04 – 0.32 µg/L
Incubation Time: 48 hours
Sample Type: Serum
Sample Size: 100 µL
For Research Use Only
Serum samples are diluted with a buffer solution. The diluted samples are added into the microtiter plate wells [PLATE] coated with Lactobacillus rhamnosus which metabolizes folic acid. The addition of folic acid in either standards [STD] or samples gives a folic acid-dependent growth response until it is consumed. After incubation at 37°C for 48 hours, the growth of Lactobacillus rhamnosus is measured turbidimetrically at 610-630 nm (alternative at 540-550 nm) in an ELISA-reader and a standard curve is generated from the dilution series. The amount of folic acid is directly proportional to the turbidity.
Lowered folic acid levels occur because of:
• a decreased supply (e.g.through alcoholism or folic acid antagonists),
• a disrupted resorption (e.g.in celiac disease, CED),
• an increased requirement (e.g.during pregnancy,in anaemic or cancerous diseases).
Symptoms of Deficiency
The first symptoms of deficiency are weariness, irritability, concentration problems and loss of appetite; further consequences are inflammation of the mucous membranes, anaemia and grievous neurological damage. During pregnancy – when the folic acid requirement sare doubled – a deficiency in folic acid may lead to premature birth and severe abnormalities.An optimal supplementation of folic acid during the pregnancy can reduce the risk of neural tube defects in the foetus by 85%. Because a deficiency of either vitamin B12 or folic acid may lead to megalobastic anaemia,the determination of both vitamins is important for the clinical picture so that the correct vitamin may be supplemented.Otherwise,in the case of vitamin B12 deficiency,treatment of megaloblastic anaemia with folic acid may lead to irreversible damage of the central nervous system.