Vitamin B12 Microtiter Plate Assay


The Vitamin B12 Assay Kit is a microtiter plate test kit based on a microbiological assay which measures the total Vitamin B12 content in serum. The test kit contains all required reagents, e.g. standard, medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the Vitamin B12 content.  The Vitamin B12 Assay Kit is for research use only.

SKU: KIFR012 Categories: , ,

Vitamin B12 Microtiter Plate Assay

Vitamin B12 Microtiter Plate Assay manufactured in Germany by Immundiagnostik

Size: 1×96 wells
Sensitivity: 6 ng/L
Dynamic Range: 6 – 54 ng/L
Incubation Time: 48 hours
Sample Type: Serum
Sample Size: 100 µL
Alternative Names: Cobalamin
For Research Use Only
Controls Included

Assay Principle

The serum is pre-treated and diluted with a buffer mixture, and then samples were transferred in the wells of a microtiter plate [PLATE] coated with Lactobacillus delbrueckii subsp. lactis. The addition of vitamin B12 in either standards [STD] or samples gives a vitamin B12-dependent growth response until vitamin B12 is consumed. After incubation at 37°C for 44 – 48 hours, the growth of Lactobacillus plantarum is measured turbidimetrically at 610 – 630 nm (alternatively at 540 – 550 nm) in an ELISA-reader and a standard curve is generated from the dilution series. The amount of vitamin B12 is directly proportional to the turbidity.

Vitamin B12-Deficiency
Vitamin B12 deficiency is seldom caused by dietary factors.In most cases, it results from a resorption disorder of the intestines or defective development of intrinsic factor. Since vitamin B12 resorption could be reduced up to 50% in the elderly, an increased supplement is recommended. Pregnant women with alacto-vegetarian diet are also recommended to increase the intake because their liver vitamin B12 stores may be exhausted. The classical vitamin B12 deficiency disease is pernicious anemia. In the early stages of the disease, vitamin B12 deficiency symptoms are manifested as weariness, palpitations, pallor or jaundice.

Indications for Vitamin-B12-determination
– Megaloblastic (pernicious) anemia
– Hyperhomocysteinaemia (patients on dialysis, old people)
– Homocysteinurie
– Peripheral neuropathy
– Patients with CED, gastritis, gastrektomy, gluten intolerance or intestinal resorption
disorders,-pancreatic insufficiency
– Patients with thrombosis
– Alcoholism
– Chronic liver and kidney disease
– Vitamin B12 deficiency from diet (vegan vegetarians)
– Pregnancy and lactation

Related Products

Vitamin B1 Microtiter Plate Assay Kit
Vitamin B2 Serum Microtiter Plate Assay Kit
Vitamin B6 (Pyridoxin) Microtiter Plate Assay Kit

Additional Information

Assay Background

Vitamin B12 (Cobalamin), a collective term for a group of variously substituted corrinoid with cobalt as the central atom, is found free and also protein-bound in food. The protein-bound form is degraded by pancreatic protease, releasing free B12 which binds to intrinsic factor, a protein secreted by gastric parietal cells of the stomach mucosa. The Cobalamin-Intrinsic-Factor-Complex is bound to mucous membrane cells of the stomach in the ileum and absorbed by the cells. In the case of high doses, a diffusion of the complex also takes place. Vitamin B12 is bound to the protein Transcobalamin II (TC-II) within the cells. TC-II serves as a transport protein for Vitamin B12 in the circulation system. Vitamin B12 is involved in the metabolism process as a co-enzyme and plays an important role in the formation of the blood, the development of the nervous system and the regeneration of the mucous membranes. In addition, there is a direct relationship to the formation of folic acid because methylcobalamin is involved in the transfer of methyl groups for the synthesis of methionine from homocysteine.

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations