Vitamin B2 Whole Blood Microtiter Plate Assay Kit


The Vitamin B2 Whole Blood Assay is a microtiter plate test kit based on a microbiological assay which measures the total Vitamin B2 content in EDTA whole blood. The test kit contains all required reagents, e.g. standard, medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the Vitamin B2 content. For use in human and veterinary medicine and in research.

SKU: KIFR002VB Categories: , ,

Vitamin B2 Whole Blood Microtiter Plate Assay Kit

Vitamin B2 Whole Blood Microtiter Plate Assay Kit manufactured in Germany by Immundiagnostik

Size: 1×96 wells
Sensitivity: 5 µg/L
Dynamic Range: 5 – 60 µg/L
Incubation Time: 48 hours
Sample Type: Whole Blood
Sample Size: 100 µL
For Research Use Only
Controls Included

Preparation and Storage:

  • Store test kit / reagents at 2-8°C.
  • Prepare reagents freshly and use immediately after preparation. Discard remaining unused reagents and waste in accordance with country, federal, state, and local regulations.
  • Put unused reagents(standard,medium)in the test kit and store at 2-8°C.
  • Store unused strips in the original package with dry bag securely closed at 2-8° C to prevent contamination or moisture exposure.
  • No warranty can be given after the expiry date (see label of test package).
  • To run assay more than once, ensure that reagents are stored at conditions stated on the label. Prepare only the appropriate amount necessary for each assay.The kit can be used up to 4 times within the expiry date stated on the label.

Assay Principle

Pretreated EDTA whole blood samples are transferred in the wells of a microtiter plate [PLATE] coated with Lactobacillusrhamnosus. The addition of Vitamin B2 in either standards [STD] or samples give a Vitamin B2-dependent growth response until Vitamin B2 is consumed. After incubation at 37°C for 48 hours, the growth of Lactobacillusrhamnosus is measured turbidimetrically at 610 – 630nm (alternatively at 540 – 550nm) in an ELISA-reader and a standard curve is generated from the dilution series. The amount of Vitamin B2 is directly proportional to the turbidity.

Related Products

Vitamin B2 Serum Microtiter Plate Assay
Vitamin B6 Microtiter Plate Assay
Vitamin B1 Microtiter Assay Kit

Additional Information

Assay Background

Vitamin B2 is a component of the flavin-nucleotides and as such is involved in the transport of hydrogen ions and electrons. It is an essential coenzyme for the metabolism of carbohydrates, fats, and proteins. The biological form of vitamin B2 is riboflavin-5-phosphate. The most important vitamin B2 derivatives are flavin mononucleotide (FMN) and flavin-adenine dinucleotide (FAD). In blood, about 60% of FAD and FMN are protein bound; only about 0.5 – 2% occur in free form.Riboflavin-5-phosphate, FMN, and FAD are transported in the plasma by a variety of proteins, including albumin, fibrinogen,riboflavin-binding protein and other globulins. Although vitamin B2 is eliminated regularly in the urine, its determination in urine samples is not recommended because of fluctuations in the concentration.

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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