Urinary Isoprostane ELISA Assay
The Urinary Isoprostane ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.05 ng/ml
Dynamic Range: 0.05 – 100 ng/ml
Incubation Time: 2.5 hour
Sample Type: Urine
Sample Size: 100 µl
Product manufactured in the USA
This Urinary Isoprotane ELISA Assay kit is a competitive enzyme-linked immunoassay (ELISA) for determining levels of 15-isoprostane F2t (the best characterized isoprostane) in urine samples. Briefly, urine samples are mixed with an enhanced dilution buffer that essentially eliminates interference due to non-specific binding. The 15-isoprostane F2t in the samples or standards competes with 15-isoprostane F2t conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for 15-isoprostane F2t coated on the microplate. The HRP activity results in color development when substrate is added, with the intensity of the color proportional to the amount of 15-isoprostane F2t-HRP bound and inversely proportional to the amount of unconjugated 15-isoprostane F2t in the samples or standards.
Using the specially formulated buffers and diluents provided, one can simply dilute urine samples and perform the Isoprostane ELISA assay. No solid phase extraction is required! Results obtained using this Urinary Isoprostane ELISA kit have been extensively validated by GC/MS following solid phase extraction of separate aliquots and show a very high degree of correlation (r > 0.8).