Lipid Peroxidase Assay Kit
The Lipid Peroxidase Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.1 nmol/mL
Dynamic Range: 0.5 – 15 µM
Incubation Time: 3 hours
Sample Type: Biological Fluids
Sample Size: 140 µl
Alternative Name: LPO
Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides are unstable and decompose to form a complex series of compounds including reactive carbonyl compounds. Polyunsaturated fatty acid peroxides generate malondialdehyde (MDA) and 4-hydroxyalkenals (HAE) upon decomposition. The measurement of MDA and HAE has been used as an indicator of lipid peroxidation (1).
Unless assayed immediately, samples should be frozen at -70°C to prevent loss of MDA (3,4) and prevent new sample oxidation. Samples should not be stored at -20°C. Once thawed from -70°C storage for assay, the sample should not be refrozen. Samples should be protected from light to avoid photoxidation.
We recommend adding BHT at a final concentration of 5 mM in the buffer prior to homogenization of tissue or cells. If no antioxidant is added, new lipid peroxidation can occur during homogenization and biased values will result (2).
Plasma or Serum:
The amount of free MDA in normal plasma or serum is at or below the limit of detection of this assay.
Cells cultured in serum containing medium should be washed several times to remove serum components prior to homogenization. Since MDA exists as the water-soluble enolate anion at physiological pH, much of the MDA generated from lipid peroxidation in cell culture may be in the culture medium.