Lipoxin A4 ELISA Assay

$815.00

The Lipoxin A4 ELISA Assay is for the quantitative determination of Lipoxin A4 in biological fluids by a microplate enzyme immunoassay (ELISA).

SKU: LA439-K01 Categories: , ,

Lipoxin A4 ELISA Assay

The Lipoxin A4 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.02 ng/ml
Dynamic Range: 0.02 – 20 ng/ml
Incubation Time: 2 hour
Sample Type: Biological Fluids
Sample Size: 100 µl

Product manufactured in the USA


Assay Background

Lipoxin A4 (LXA4) is a biologically active lipoxygenase interaction product derived from arachidonic acid. Arachidonic acid is first oxygenated by 15-lipoxygenase to form 15-HETE which is converted by 5-lipoxygenase and epoxide hydrase to generate LXA4. LXA4 stimulates leukocyte chemotaxis without aggregation and inhibits natural killer cell cytotoxicity. It also provokes contraction of parenchymal strips and stimulates microvascular changes. Recent findings indicate that LXA4 inhibits leukocyte-dependent inflammation. Determination of LXA4 level may provide new understanding of the role of LXA4 in basic cellular reactions and in pathophysiology of inflammation and other disease processes.


CROSS REACTIVITY
Lipoxin A4 100.0%
15-epi- Lipoxin A4 24%
5(S)6(R)-Di-HETE 5.00%
Lipoxin B4 1.00%
15-HETE 0.10%
5-HETE <0.10% 12-HETE <0.10% Leukotriene B4 <0.01% Leukotriene C4 <0.01% Leukotriene D4 <0.01% Leukotriene E4 <0.01%


STORAGE
1. Store the components of this kit at the temperatures specified on the labels.
2. Unopened reagents are stable until the indicated kit expiration date.
3. Concentrated, reconstituted conjugate has a shelf life of at least two weeks when stored properly.
4. Desiccant bag must remain in foil pouch with unused strips. Keep pouch sealed when not in use to maintain a dry environment. Remove excess air before sealing.


Related Products

15-Epi-Lipoxin A4 ELISA Assay Kit
Anti-8-Lipoxygenase Polyclonal Antibody
15-Isoprostane F2t ELISA Assay Kit

Additional Information

Assay Principle


Lipoxin A4 ELISA Assay kit (Enzyme-Linked ImmunoSorbent Assay) is for the quantitative analysis of Lipoxin A4 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the LXA4 in the sample for a limited number of antibody binding sites.

The sample or standard solution is first added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate that generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650 nm. The extent of color development is inversely proportional to the amount of LXA4 in the sample or standard. For example, the absence of LXA4 in the sample will result in a bright blue color, whereas the presence of LXA4 will result in decreased or no color development.

  1. Add 50 µL of Standards or Samples (may require diluting) to the corresponding wells on the microplate in duplicate.
  2. Add 50 µL of diluted LXA4-HRP Conjugate to each well. Incubate at room temperature for one hour.
  3. Wash the plate three times with 300 µL of diluted Wash Buffer per well. Wash 5 times if using an automated plate washer.
  4. Add 150 µL of TMB Substrate to each well. Incubate at room temperature for 30 minutes.
  5. Read the plate at 650 nm.

Cross Reactivity Data


Lipoxin A4 100.0% 12-HETE <0.10%
15-epi- Lipoxin A4 24% Leukotriene B4 <0.01%
5(S)6(R)-Di-HETE 5.00% Leukotriene C4 <0.01%
Lipoxin B4 1.00% Leukotriene D4 <0.01%
15-HETE 0.10% Leukotriene E4 <0.01%
5-HETE <0.10%

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