GST Alpha ELISA Assay

$790.00

The GST Alpha ELISA Assay is for the quantitative determination of Glutathione S-Transferase (GST) Alpha in human serum by a microplate enzyme immunoassay (ELISA).

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GST Alpha ELISA Assay

The GST Alpha ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.1 ng/ml
Dynamic Range: 0.1 – 10 ng/ml
Incubation Time: 2 hour
Sample Type: Serum
Sample Size: 100 µl
Species: Human

Product manufactured in the USA


Assay Background

Glutathione S-Transferase (GST) has multiple isoforms. This assay is specific for Glutathione S-Transferase Alpha (GSTA) and is not known to cross react with the mu, pi, or theta variants. GSTA is a common biomarker for hepatocellular damage. It also conjugates GSH to 4-hydroxynonenal, a product of lipid peroxidation and is an important player in cellular antioxidant defense mechanisms.


STORAGE
1. Store the components of this kit at the temperatures specified on the labels.
2. Unopened reagents are stable until the indicated kit expiration date.

SAMPLE STORAGE
Samples should be stored at –80°C and thawed just prior to use. Avoid repeated freeze/thaw cycles for best results. This assay was developed and validated with human serum samples, however it does cross-react with rat.
SAMPLE PREPARATION
It is recommended to do multiple sample dilutions to ensure that the concentration falls within the accepted range for the assay. Samples should be assayed neat or diluted 1:2 in Assay Buffer.


Related Products

Rat GST alpha ELISA
GST Pi Assay Kit

Additional Information

Assay Principle


This Glutathione S-Transferase (GST) Alpha ELISA Assay kit is a standard sandwich enzyme-linked immunosorbent assay (ELISA). The plate is pre-coated with anti-GSTA and blocked, ready for the addition of samples and standards. The assay should take approximately 3 hours to run, plus any required sample preparation time.

  1. Add 100 µL of Standards and Samples to the corresponding wells on the microplate in duplicate. Incubate at room temperature for one hour.
  2. Dump the contents of the plate and wash each well three times with 300 µL of Wash Buffer. After the final wash, tap the plate on a lint-free paper towel to make sure there is no solution left in the wells.
  3. Add 100 µL of the Detection Antibody to each well. Incubate at room temperature for one hour.
  4. Wash the plate as in step 2.
  5. Add 100 µL of the HRP Conjugate to each well. Incubate at room temperature for 30 minutes.
  6. Wash the plate as in step 2.
  7. Add 100 µL of TMB Substrate to each well. Allow the color to develop for 20-30 minutes at room temperature.
  8. Stop the reaction by adding 25 µL per well of 3N Sulfuric Acid (H2SO4).
  9. Read the plate at 450 nm in a microplate reader.

Manual

Product Manual


Publications

References


Batistaki, C;Michalopoulos, G;Matsota, P;Nomikos, T;Kalimeris, K;Riga, M;Nakou, M;Kostopanagiotou, G;, CYP2E1 immunoglobulin G4 subclass antibodies after desflurane anesthesia, World Journal of Hepatol, 2014, Volume 6, Issue 5, Page 340-6, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033291/

Product Citations