8-Isoprostane ELISA Assay Kit
The 8-Isoprostane ELISA Assay Kit is For Research Use Only and manufactured in the USA
Size: 1×96 wells
Sensitivity: 10 pg/ml
Dynamic Range: 10 -5000 pg/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma, Tissue, Cell Culture
Sample Size: 100 µl
Storage and Stability
This 8-Isoprostane ELISA will obtain optimal results if all of the components are stored at the proper temperature prior to use. Items should be stored at the designated temperatures upon receipt of this kit.
All components of the kit are stored below -20°C and should not be re-frozen and thawed more than necessary.
The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissues phospholipids by oxygen radicals. A recent NIH-sponsored study on Biomarkers of Oxidative Stress has indicated that 8-isoprostane is the best index of oxidative injury in a well-accepted oxidant stress rat model (1,2). In addition, plasma 8-isoprostane levels were found to be elevated in elderly subjects with severe hypertension (3) and in urine from subjects with high fat diet-induced liver steatosis (4).
This 8-Isoprostane ELISA Assay kit can be used for the determination of 8-isoprostane in diluted urine, serum, plasma, cells, and tissues following proper isolation and purification of the eicosanoid from the isoprostane-containing sample.