Steroid ELISA Assay

Triiodothyronine T3 ELISA Assay Kit

$225.00

The Triiodothyronine ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the direct quantitative determination of triiodothyronine (T3) in human serum. The Triiodothyronine (T3) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: T3T31-K01 Categories: , ,

Triiodothyronine T3 ELISA Assay Kit

The Triiodothyronine T3 ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.16 ng/mL
Dynamic Range: 0.2–10 ng/mL
Incubation Time: 75 minutes
Sample Type: Serum
Sample Size: 50 μL
Controls Included

Assay Principle

The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the color formed is inversely proportional to the concentration of T3 in the sample. A set of standards is used to plot a standard curve from which the amount of T3 in samples and controls can be directly read.

SPECIMEN COLLECTION AND STORAGE
Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.

Related Products

Free Triiodothyronine (T3) ELISA
Reverse T3 (rT3) ELISA Assay
Free Thyroxine (fT4) ELISA Kit

Additional Information

Assay Background

Triiodothyronine (T3) and thyroxine (T4) are the two active thyroid hormones found in the blood stream. About 80% of T3 is produced by the deiodination of T4 in the peripheral tissue and the other 20% is produced directly from the thyroid gland. T3 is transported through the peripheral blood stream bound to serum proteins, namely thyroxine binding globulin, thyroid binding prealbumin and albumin. About 0.3% of the total T3 is unbound and is therefore considered the free fraction. T3 has an influence on oxygen consumption and heat production in virtually all tissues. The hormone also plays a critical role in growth, development and sexual maturation of growing organisms. T3 is one parameter used in the clinical diagnosis and differentiation of thyroid disease, particularly hyperthyroidism. In most hyperthyroid patients, both serum T3 and serum T4 levels are elevated. Serum T3 levels are a sensitive indicator of the impending hyperthyroid state often preceeding elevated T4 and free thyroxine index values. Serum T3 levels are clinically signifi cant in both the diagnosis of thyroid disease and in the detection of T3- thyrotoxicosis. However, it has been demonstrated that T3 levels may be affected by a number of medications, acute and chronic stress, and a variety of acute and chronic nonthyrodial illnesses. It is therefore necessary to differentiate those results that are due to thyroid dysfunction from those related to non-thyrodal diseases.

Typical Standard Curve

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations