Reverse T3 ELISA Assay Kit
Reverse T3 ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.009 ng/mL
Dynamic Range: 0.02–2 ng/mL
Incubation Time: 105 minutes
Sample Type: Serum, plasma
Sample Size: 25 μL
Alternative Names: Human Reverse T3 ELISA Kit, rT3 ELISA, Human rT3 ELISA
The rT3 ELISA is a competitive enzyme immunoassay, where the antigen (rT3 present in calibrators, controls and patient samples) competes with a biotin-labelled antigen (rT3-Biotin conjugate) for a limited quantity of antibody which is coated on the microplate wells. After one hour incubation followed by the first washing, unbound materials are removed and a Streptavidin-HRP conjugate is added and incubated for 30 minutes. Following a second washing, the TMB substrate is added. The enzymatic reaction is terminated by addition of the stopping solution, upon which the color intensity is measured with a microplate reader. The color intensity is inversely proportional to the concentration of rT3 in the sample. The set of kit calibrators that are run simultaneously with the samples is used to plot a calibration curve and determine the concentration of rT3 in samples and controls.
SPECIMEN COLLECTION AND STORAGE
Serum: Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower for longer time.
Plasma: Approximately 0.2 mL of plasma is required per duplicate determination. Collect 4–5 mL of blood into EDTA plasma tubes. Store at 4°C for up to 24 hours or at -10°C or lower for longer time.
Consider all human specimens as possible biohazardous materials and take precautions when handling.