5 alpha-Androstane-3 alpha 17 beta-diol Glucuronide 3 alpha-Diol G LIA
5 alpha-Androstane-3 alpha 17 beta-diol Glucuronide 3 alpha-Diol G LIA is for Research Use Only
Size: 1×96 wells
Sensitivity: 0.1 ng/mL
Dynamic Range: 0.25–50 ng/mL
Incubation Time: 60 minutes
Sample Type: Serum
Sample Size: 50 μL
The principle of the following chemiluminescence immunoassay (LIA) test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the luminescence substrate solution is added. The relative luminescence units (RLUs) are measured on a microtiter plate luminometer. The RLU values are inversely proportional to the concentration of 3α-Diol G in the sample. A set of calibrators are used to plot a standard curve from which the amount of 3α-diol G in patient samples and controls can be directly read.
SPECIMEN COLLECTION AND STORAGE
Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.