Total Estrogens ELISA Assay Kit
The Total Estrogens ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 12.4 pg/mL
Dynamic Range: 20–2,500 pg/mL
Incubation Time: 3 hours
Sample Type: Serum
Sample Size: 50 µL
A total estrogens test is commonly indicated to:
• Aid in diagnosis of sex steroid metabolism related conditions, for example, premature or delayed puberty, and aromatase and 17 alpha-hydroxylase deficiencies.
• Assess fracture risk in postmenopausal women and, to a lesser degree, older men.
• Follow-up female hormone replacement therapy in postmenopausal women.
• Prognose antiestrogen therapy, for example, aromatase inhibitor therapy.
The total estrogens ELISA is a competitive immunoassay. Competition occurs between total estrogens (estrone, estradiol, and estriol) present in standards, controls and patient samples and an enzyme-labelled antigen (conjugate) for a limiting number of anti-estrogen antibody binding sites on the microplate wells. After a washing step that removes unbound materials the enzyme substrate is added and approximately 15–20 minutes later the enzymatic reaction is terminated by addition of stopping solution. The resulting optical density (OD), measured with a microplate reader, is inversely proportional to the concentration of total estrogens in the sample. A standard curve is plotted with a provided set of standards to calculate directly the concentration of total estrogens in patient samples and controls.
SPECIMEN COLLECTION AND STORAGE
Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.