Total Estrogens ELISA Assay Kit
The Total Estrogens ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 12.4 pg/mL
Dynamic Range: 20–2,500 pg/mL
Incubation Time: 3 hours
Sample Type: Serum
Sample Size: 50 µL
Assay Background for Total Estrogens ELISA
Total estrogens comprise the total quantity of estrone, estradiol, and estriol. The estrogens are involved in the development of female sex organs and secondary sex characteristics. Before the ovum is fertilized the main action of the estrogens is on the growth and function of the reproductive tract to prepare it for the fertilized ovum. During the follicular phase of the menstrual cycle the total estrogens level shows a slight increase. The production of total estrogens then increases markedly to peak at around day 13. The peak is of short duration and by day 16 of the cycle levels will be low. A second peak occurs at around day 21 of the cycle; if fertilization does not occur, the production of total estrogens decreases.
In post-menopausal women the concentration of all estrogens decreases substantially and estrone becomes the predominant estrogen. In pregnant women the concentration of all estrogens escalates and estriol becomes the predominant estrogen.
A total estrogens test is commonly indicated to:
• Aid in diagnosis of sex steroid metabolism related conditions, for example, premature or delayed puberty, and aromatase and 17 alpha-hydroxylase deficiencies.
• Assess fracture risk in postmenopausal women and, to a lesser degree, older men.
• Follow-up female hormone replacement therapy in postmenopausal women.
• Prognose antiestrogen therapy, for example, aromatase inhibitor therapy.
SPECIMEN COLLECTION AND STORAGE
Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.