Total Estrogens ELISA Assay Kit


The Total Estrogens ELISA Assay Kit is intended for the quantitative determination of Total Estrogens in human serum. The Total Estrogens ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: ESG31-K01 Categories: , ,

Total Estrogens ELISA Assay Kit

The Total Estrogens ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 12.4 pg/mL
Dynamic Range: 20–2,500 pg/mL
Incubation Time: 3 hours
Sample Type: Serum
Sample Size: 50 µL
Controls Included

A total estrogens test is commonly indicated to:
• Aid in diagnosis of sex steroid metabolism related conditions, for example, premature or delayed puberty, and aromatase and 17 alpha-hydroxylase deficiencies.
• Assess fracture risk in postmenopausal women and, to a lesser degree, older men.
• Follow-up female hormone replacement therapy in postmenopausal women.
• Prognose antiestrogen therapy, for example, aromatase inhibitor therapy.

Assay Principle

The total estrogens ELISA is a competitive immunoassay. Competition occurs between total estrogens (estrone, estradiol, and estriol) present in standards, controls and patient samples and an enzyme-labelled antigen (conjugate) for a limiting number of anti-estrogen antibody binding sites on the microplate wells. After a washing step that removes unbound materials the enzyme substrate is added and approximately 15–20 minutes later the enzymatic reaction is terminated by addition of stopping solution. The resulting optical density (OD), measured with a microplate reader, is inversely proportional to the concentration of total estrogens in the sample. A standard curve is plotted with a provided set of standards to calculate directly the concentration of total estrogens in patient samples and controls.

Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.

Related Products

Estrone ELISA Assay Kit
Estradiol ELISA Assay Kit
Testosterone ELISA Assay Kit

Additional Information

Assay Background

Total estrogens comprise the total quantity of estrone, estradiol, and estriol. The estrogens are involved in the development of female sex organs and secondary sex characteristics. Before the ovum is fertilized the main action of the estrogens is on the growth and function of the reproductive tract to prepare it for the fertilized ovum. During the follicular phase of the menstrual cycle the total estrogens level shows a slight increase. The production of total estrogens then increases markedly to peak at around day 13. The peak is of short duration and by day 16 of the cycle levels will be low. A second peak occurs at around day 21 of the cycle; if fertilization does not occur, the production of total estrogens decreases.

In post-menopausal women the concentration of all estrogens decreases substantially and estrone becomes the predominant estrogen. In pregnant women the concentration of all estrogens escalates and estriol becomes the predominant estrogen.

Typical Standard Curve

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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