Ferritin ELISA Assay Kit
The Ferritin ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.44 ng/mL
Dynamic Range: 10–800 ng/mL
Incubation Time: 45 minutes
Sample Type: Serum
Sample Size: 20 µL
Alternative Names: Human Ferritin ELISA
Assay Principle for Ferritin ELISA
The principle of the Ferritin ELISA Assay Kit enzyme immunoassay test follows a typical one-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for ferritin is immobilized onto the microplate and another monoclonal antibody specific for a different region of ferritin is conjugated to horse radish peroxidase (HRP). Ferritin from the sample and standards are allowed to bind simultaneously to the plate and to the HRP conjugate. The washing and decanting steps remove any unbound HRP conjugate. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of ferritin in the sample. A set of standards is used to plot a standard curve from which the amount of ferritin in patient samples and controls can be directly read.
SPECIMEN COLLECTION AND STORAGE
Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.