Ferritin ELISA Assay Kit

$190.00

The Ferritin ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of Ferritin in human serum. The Ferritin ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: FRR31-K01 Categories: , ,

Ferritin ELISA Assay Kit

The Ferritin ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.44 ng/mL
Dynamic Range: 10–800 ng/mL
Incubation Time: 45 minutes
Sample Type: Serum
Sample Size: 20 µL
Alternative Names: Human Ferritin ELISA
Controls Included


Assay Principle for Ferritin ELISA

The principle of the Ferritin ELISA Assay Kit enzyme immunoassay test follows a typical one-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for ferritin is immobilized onto the microplate and another monoclonal antibody specific for a different region of ferritin is conjugated to horse radish peroxidase (HRP). Ferritin from the sample and standards are allowed to bind simultaneously to the plate and to the HRP conjugate. The washing and decanting steps remove any unbound HRP conjugate. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of ferritin in the sample. A set of standards is used to plot a standard curve from which the amount of ferritin in patient samples and controls can be directly read.


SPECIMEN COLLECTION AND STORAGE
Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.


Related Products

Human Leptin ELISA Assay Kit
B2-Microglobulin ELISA Assay Kit
Mouse Ferritin ELISA Kit

Additional Information

Assay Background


Ferritin is a protein which serves as a storage center for iron. It is found in many tissues but the highest concentrations are in the liver, spleen and bone marrow. The total body iron stores in normal people correlate well with the concentration of ferritin in serum. If there is a deficiency in iron, that is the concentration of iron is low in the blood, the ferritin result will be decreased. Likewise, an overload of iron indicates an increase in the level of ferritin. However, in some conditions like liver disease ferritin will be elevated.

Typical Standard Curve


Documents


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.