Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) ELISA Assay Kit
The Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 31.2pg/ml
Standard Range: 31.2pg/ml – 2000pg/ml
Incubation Time: 3 hrs 35 mins
Sample Type: Serum, plasma, and cell culture supernates
Sample Size: 100 µl
Assay Principle
Th Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human TIMP-1. Standards and samples are pipetted into the wells and any human TIMP-1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human TIMP-1 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human TSP-2 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human TIMP-1, the unknown sample concentration can be interpolated from a reference curve included in each assay.
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