Autotaxin ELISA Assay Kit

$490.00

The Autotaxin ELISA Assay Kit is designed for quantification of human autotaxin in serum, plasma, and fluid. The Eagle Biosciences Autotaxin ELISA Assay Kit is for research use only and not to be used for diagnostic procedure.

SKU: ATX31-K01 Categories: , ,

Autotaxin ELISA Assay Kit

The Autotaxin ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.78ng/mL
Standard Range: 1.56ng/mL-50ng/mL
Incubation Time: 2 hours 35 minutes
Sample Type: Serum, plasma, and fluid
Sample Size: 100 µl
Alternative Names: ATX, ENPP2


Assay Principle

The Autotaxin assay is a quantitative sandwich ELISA. The micro-plate is pre-coated with a rabbit polyclonal antibody against human autotaxin. Standards and samples are pipetted into the wells and any human autotaxin present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human autotaxin is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and color develops in proportion to the amount of human autotaxin bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human autotaxin, the unknown sample concentration can be interpolated from a reference curve included in each assay.


Related Products

AFP ELISA Assay Kit
Rat TNF-Alpha ELISA Assay Kit
Mouse Neprilysin ELISA Assay Kit

Additional Information

Assay Background


Autotaxin, also known as ENPP-2, is a secreted glycoprotein which belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Generally, NPPs can hydrolyze phosphates from nucleotides. Autotaxin exhibits the unique lysophospholipase D activity. The mature protein includes two somatomedin-B-like (SMB) cysteine knot domains, a catalytic domain, and an inactive C-terminal nuclease-like domain with an EF-hand-like motif that is important in cell motility, and a region involved in autotaxin secretion. There are three isoforms identified in mouse and human. Most circulating autotaxin is the β form which contains 863 amino acid. Autotaxin contributes to the predominant extracellular source of the phospholipid LPA (lysophosphatidic acid) from LPC (lysophosphatidylcholine). Autotaxin can also produce minor amounts of sphingosine 1-phosphate and cyclic phosphatidic acid which can antagonize many of the tumorigenic properties of LPA.
Autotaxin stimulates tumor cell motility and enhances invasion and metastasis. It’s upregulated in melanoma, glioblastoma, breast and lung carcinoma, follicular lymphoma and other cancers. Autotaxin production by adipocytes enhances pre-adipocyte proliferation and may be elevated in obesity. Autotaxin is present in blood, urine, saliva, seminal and cerebrospinal fluids. In addition, plasma autotaxin is cleared by the liver which is elevated in liver disease. Normal serum or plasma autotaxin concentration is reported to be slightly higher in females than in males, and highest in pregnant females.

Documents

Product Manual