Mouse Proteinase 3 (PR3) ELISA Assay Kit
The Mouse Proteinase 3 (PR3) ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.078ng/ml
Standard Range: 0.1ng/ml – 6.4ng/ml
Incubation Time: 2 hrs 15 minutes
Sample Type: Serum, plasma, and other biological fluid
Sample Size: 100 µl
Assay Principle
The Mouse Proteinase 3 (PR3) assay is a quantitative sandwich ELISA. The microtiter strips are pre-coated with a polyclonal antibody specific for mouse PR3. Standards and samples are pipetted into the wells and any mouse PR3 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidise (HRP) labelled polyclonal antibody specific for mouse PR3 is added to the wells. After wash step to remove any unbound reagents, an HRP substrate solution is added and colour develops in proportion to the amount of mouse PR3 bound initially. The assay is stopped and the optical density of the wells is determined using a micro-plate reader. Since the increase in absorbance is directly proportional to the amount of captured mouse PR3, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Sample Preparation
Serum or plasma sample is generally required a 50-fold dilution in 1×Assay buffer. A suggested dilution step is to add 10 µl of sample to 490 µl of 1×Assay buffer. If a sample has a PR3 level greater than the highest standard, the sample should be diluted further and the assay should be repeated.
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