Mouse Proteinase 3 (PR3) ELISA Assay Kit


The Mouse Proteinase 3 (PR3) ELISA Assay kit is designed for quantification of mouse PR3 in serum, plasma, cell culture media and other biological samples. The Eagle Biosciences Mouse Proteinase 3 ELISA kit is for research use only and not to be used for diagnostic procedures.

SKU: PRT11-K01 Categories: , ,

Mouse Proteinase 3 (PR3) ELISA Assay Kit

The Mouse Proteinase 3 (PR3) ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.078ng/ml
Standard Range: 0.1ng/ml – 6.4ng/ml
Incubation Time: 2 hrs 15 minutes
Sample Type: Serum, plasma, and other biological fluid
Sample Size: 100 µl

Assay Principle

The Mouse Proteinase 3 (PR3) assay is a quantitative sandwich ELISA. The microtiter strips are pre-coated with a polyclonal antibody specific for mouse PR3. Standards and samples are pipetted into the wells and any mouse PR3 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidise (HRP) labelled polyclonal antibody specific for mouse PR3 is added to the wells. After wash step to remove any unbound reagents, an HRP substrate solution is added and colour develops in proportion to the amount of mouse PR3 bound initially. The assay is stopped and the optical density of the wells is determined using a micro-plate reader. Since the increase in absorbance is directly proportional to the amount of captured mouse PR3, the unknown sample concentration can be interpolated from a reference curve included in each assay.

Sample Preparation

Serum or plasma sample is generally required a 50-fold dilution in 1×Assay buffer. A suggested dilution step is to add 10 µl of sample to 490 µl of 1×Assay buffer. If a sample has a PR3 level greater than the highest standard, the sample should be diluted further and the assay should be repeated.

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Additional Information

Assay Background

Proteinase 3 (PR3), also known as myeloblastin, Wegener autoantigen, PRTN3 and NP- 4, is one of the hematopoietic serine proteases localized in the primary granules of polymorphonuclear neutrophils (PMNs). The primary function of PR3 is recognized as to participate in direct intracellular killing of phagocytosed pathogens in phagolysosomes and degradation of extracellular matrix components at inflammatory sites. PR3 has also been proven to be able to process some pro-inflammatory cytokines, such as IL-1β, IL-18 and TNF-α, activate mitogen activated protein kinase (MAPK) signaling through proteinase activated receptor-1 (PAR1), and induce endothelial cell apoptosis through NF-κB signaling pathways. PR3 is identified as the target autoantigen of anti-neutrophil cytoplasmic autoantibodies (ANCA) in Wegener granulomatosis. Increased PR3 levels have been reported in patients with acute myocardial infarction, and in subjects with type 1 diabetes.

Typical Standard Curve

Mouse Proteinase 3 (PR3) ELISA Standard Curve


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