Thyroxine (T4) ELISA Assay


The Thyroxine ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the direct quantitative determination of thyroxine (T4) in human serum. The Eagle Biosciences Thyroxine (T4) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: T4T31-K01 Categories: , ,

Thyroxine T4 ELISA Assay

Thyroxine T4 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.6 μg%
Dynamic Range: 1–32 μg%
Incubation Time: 50 minutes
Sample Type: Serum
Sample Size: 20 μL
Controls Included

Assay Principle

The principle of the Thyroxine ELISA Assay Kit enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of T4 in the sample. A set of standards is used to plot a standard curve from which the amount of T4 in patient samples and controls can be directly read.

Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
Human serum that may be used in the preparation of the standards and controls has been tested and found to be nonreactive for Hepatitis B surface antigen and has also been tested for the presence of antibodies to HCV and Human Immunodeficiency Virus (HIV) and found to be negative. No test method however, can offer complete assurance that HIV, HCV and Hepatitis B virus or any infectious agents are absent. The reagents should be considered a potential biohazard and handled with the same precautions as applied to any blood specimen.

Related Products

Free Thyroxine (fT4) ELISA
Triiodothyronine T3 ELISA Assay
Free Thyroxine (fT4) LIA Assay

Additional Information

Assay Background

Clinical Trends:
• The level of T4 in hypothyroid patients is decreased.
• The level of T4 in hyperthyroid patients is increased.
• In euthyroid individuals the level of T4 is within the normal range.
Thyroid binding globulin (TBG) levels have reportedly been elevated by the following conditions: increased estrogens from oral contraceptives, androgens, glucocorticords and pregnancy. Consequently, borderline T4 values should be viewed with caution when any of the above conditions are present.

Package Inserts


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