Thyroid Stimulating Hormone ELISA


The Thyroid Stimulating Hormone ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the direct quantitative determination of thyroid stimulating hormone in human serum. The Thyroid Stimulating Hormone (TSH) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: THH31-K01 Categories: ,

Thyroid Stimulating Hormone ELISA

The Thyroid Stimulating Hormone ELISA is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.1 μIU/mL
Dynamic Range: 0.2–30 μIU/mL
Incubation Time: 105 minutes
Sample Type: Serum
Sample Size: 50 μL
Alternative Names: TSH ELISA, Human Thyroid Stimulating Hormone ELISA, Human TSH ELISA
Controls Included

Assay Background

Thyroid stimulating hormone (TSH) is a glycoprotein hormone secreted by the anterior pituitary gland. TSH has two subunits, namely α and β. The α subunit of TSH is similar to the α subunit found in the LH, FSH and hCG glycoprotein hormones. The β subunit however, is specific and differs from hormone to hormone. The thyroid hormones are secreted and produced by the thyroid gland. The production of thyroid hormones is under the regulation of TSH. Also, TSH acts as a stimulator of iodide transport and the gland itself is under the positive control of TSH. The concentrations of thyroid hormones control the secretion of TSH, therefore, a negative feedback exists. It is to be noted that the secretion of thyroid hormones are under the direct, positive effect of the sympathetic nervous system. The major protein component of the thyroid gland is thyroglobulin, a glycoprotein of which the secretion in the blood stream is stimulated by TSH. Therefore, TSH plays an important role in the proper function and development of the thyroid gland. It is recommended to assay both the glycoprotein hormone and the target organ hormones. For example, in primary hypothyroidism the serum level of thyroxine is low while the TSH level is high. In secondary hypothyroidism, both thyroxine and TSH are low. The TSH level is decreased in hyperthyroidism. Today, with all the sensitive assays available, if there were to be only one test to be prescribed for thyroid function, TSH would be the test. TSH determinations are also helpful to monitor patients who receive thyroxine replacement therapy.

Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.

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Additional Information

Assay Principle

The principle of the following enzyme immunoassay test follows a typical one-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for TSH is immobilized onto the microplate and another monoclonal antibody specific for a different region of TSH is conjugated to horse radish peroxidase (HRP). TSH from the sample and standards are allowed to bind simultaneously to the plate and to the HRP conjugate. The washing and decanting steps remove any unbound HRP conjugate. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of TSH in the sample. A set of standards is used to plot a standard curve from which the amount of TSH in patient samples and controls can be directly read.

Typical Standard Curve

TSH ELISA Standard Curve




Khalid M. Khan, Faruque Parvez, R. Thomas Zoeller, Barbara A. Hocevar, Lisa M. Kamendulis, Diane Rohlman, Mahbubul Eunus, Joseph Graziano,Thyroid hormones and neurobehavioral functions among adolescents chronically exposed to groundwater with geogenic arsenic in Bangladesh,Science of The Total Environment, Volume 678, 2019, Pages 278-287, ISSN 0048-9697,