SHBG ELISA Assay Kit

$250.00

The SHBG ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of Sex Hormone Binding Globulin by an enzyme immunoassay in human serum. The Eagle Biosciences Sex Hormone Binding Globulin (SHBG) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: SHB31-K01 Categories: , ,

SHBG ELISA Assay Kit

The SHBG ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.1 nmol/L
Dynamic Range: 3.3–295 nmol/L
Incubation Time: 1 hour
Sample Type: Serum
Sample Size: 10 µL
Alternative Names:: Sex Hormone Binding Globulin
Controls Included


Assay Background

Sex hormone binding globulin (SHBG) is a glycoprotein composed of 373 amino acid residues and three carbohydrate side chains. SHBG has been known by many other names including Testosteroneestradiol Binding Globulin (TeBG), Sex steroid Binding Protein (sBP) and Sex Steroid Binding Globulin (SSBG). One of the main properties of SHBG is its high affinity for steroids, especially the C18, C19 and 17α-hydroxyl groups. The binding of steroids to SHBG is temperature and pH dependent. The three steroids that have a high avidity for SHBG are Dihydrotestosterone, Testosterone and Estradiol. Very small amounts of these steroids are free in biological fluid; the majority are bound to SHBG and albumin. These two fractions, that is, free and bound exist in a state of dynamic equilibrium. When the level of SHBG concentration changes, a remarkable change occurs in both albumin-bound hormone and also in the free fraction.
Throughout life SHBG increases until the eighties in both sexes. During the menstrual cycle SHBG does not seem to vary appreciably, however, according to some authors the concentration of SHBG is elevated in the luteal phase. During pregnancy the level of SHBG rises rapidly until about the 30th week of gestation.


SPECIMEN COLLECTION AND STORAGE
Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.


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Additional Information

Assay Principle


The principle of the Eagle Biosciences Sex Hormone Binding Globulin (SHBG) ELISA Assay Kit follows a typical two-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for SHBG is immobilized onto the microplate and another monoclonal antibody specific for a different region of SHBG is conjugated to horse radish peroxidase (HRP). SHBG from the sample and standards are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the color formed by the enzymatic reaction is directly proportional to the concentration of SHBG in the sample. A set of standards is used to plot a standard curve from which the amount of SHBG in patient samples and controls can be directly read.

Typical Standard Curve


Manual

Product Manual