Luteinizing Hormone (hLH) ELISA Assay Kit

$190.00

The Eagle Biosciences Luteinizing Hormone (hLH) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of Luteinizing Hormone in human serum. The Luteinizing Hormone (hLH) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: HLH31-K01 Categories: , ,

Luteinizing Hormone (hLH) ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.2 IU/L
Dynamic Range: 1–100 IU/L
Incubation Time: 80 minutes
Sample Type: Serum
Sample Size: 25 µL

Controls Included

Additional Information

Assay Background


Human luteinizing hormone (hLH) is a glycoprotein synthesized by the anterior lobe of the pituitary gland. This hormone consists of two subunits: α and β. The α subunit of LH is similar to the α subunit found in both the FSH and TSH glycoprotein hormones (which are also synthesized by the pituitary gland) as well as the α subunit of hCG (produced by the placenta). However, the β subunit of each of these hormones are unique. Therefore, the specificity of these four hormones are due to the β peptide chains. It is to be noted that the α chain by itself has no biological activity. The hypothalamic decapeptide, namely the gonadotropin releasing hormone (GnRH), stimulates the release of LH. Both the LH and FSH hormones in men act on the testis, which have two functions: Leydig cells secrete androgens while sperm are formed by the seminiferous tubules. The secretion of testosterone and dihydrotestosterone by the Leydig cells is under the direct control of LH.

Assay Principle


The principle of the following enzyme immunoassay test follows a typical two-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for LH is immobilized onto the microplate and another monoclonal antibody specific for a different region of LH is conjugated to horse radish peroxidase (HRP). LH from the sample and standards are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of LH in the sample. A set of standards is used to plot a standard curve from which the amount of LH in patient samples and controls can be directly read.

Typical Standard Curve


HLH Standard Curve

Manual

Product Manual