Steroid ELISA Assay

Aldosterone ELISA Assay Kit

$265.00

The Aldosterone ELISA Assay Kit is for the direct quantitative determination of Aldosterone in human serum, plasma and urine by an enzyme immunoassay.

SKU: ALD31-K01 Categories: , ,

Aldosterone ELISA Assay Kit

Aldosterone ELISA Assay Kit is for Research Use Only

Size: 1×96 wells
Sensitivity: 9.1 pg/mL
Dynamic Range: 15–1000 pg/mL
Incubation Time: 80 minutes
Sample Type: Human serum, plasma, or urine
Sample Size: 50 µL
Controls Included

Aldosterone ELISA Assay Kit INTERFERENCE
The following substances were tested and did not show significant interference in the assay:
hemoglobin up to 4 g/L, bilirubin conjugated and free up to 125 mg/L and triglycerides up to 30 mg/mL.

Assay Background for Aldosterone ELISA Assay

Aldosterone is a potent mineralocorticoid whose synthesis and release are controlled by the renin-angiotensin system of the body. Aldosterone promotes the reabsorption of sodium in the distal tubules of the kidney resulting in potassium secretion along with sodium retention, which controls the circulating blood volume. Chronic overproduction and secretion of aldosterone leads to hypertension. Aldosterone measurement levels in serum in conjunction with plasma renin activity levels can be used to differentiate between primary and secondary aldosteronism.
The measurement of aldosterone in concert with selective suppression and stimulation tests can be used to further differentiate primary aldosteronism into two basic types:
• Primary aldosteronism caused by an adenoma of one or both adrenals.
• Primary aldosteronism caused by adrenal hyperplasia.
This differentiation is vital in the treatment and management of the disease. The adrenal adenomas respond well to surgery whereas hyperplastic disease of the adrenals is generally better managed medically. In summary, the precise and accurate measurement of aldosterone by enzyme immunoassay can be an important addition to a diagnostic laboratory battery for the differential diagnosis of hypertensive disease.

Related Products to Aldosterone ELISA

Estrone ELISA Assay Kit
Estradiol ELISA Assay Kit
Ferritin ELISA Assay Kit

Additional Information

Assay Principle

The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate wells. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured with a microplate reader. The intensity of the colour formed is inversely proportional to the concentration of aldosterone in the sample. A set of standards is used to plot a standard curve from which the amount of aldosterone in patient samples and controls can be directly read.

Typical Standard Curve

Package Inserts

 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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