Aldosterone ELISA Assay Kit


The Eagle Biosciences Aldosterone ELISA Assay Kit is for the direct quantitative determination of Aldosterone in human serum, plasma and urine by an enzyme immunoassay.

SKU: ALD31-K01 Categories: , ,

Aldosterone ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 9.1 pg/mL
Dynamic Range: 15–1000 pg/mL
Incubation Time: 80 minutes
Sample Type: Human serum, plasma, or urine
Sample Size: 50 µL

Controls Included

Additional Information

Assay Background

Aldosterone is a potent mineralocorticoid whose synthesis and release are controlled by the renin-angiotensin system of the body. Aldosterone promotes the reabsorption of sodium in the distal tubules of the kidney resulting in potassium secretion along with sodium retention, which controls the circulating blood volume. Chronic overproduction and secretion of aldosterone leads to hypertension. Measurement of aldosterone levels in serum in conjunction with plasma renin activity levels can be used to differentiate between primary and secondary aldosteronism.

Assay Principle

The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate wells. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured with a microplate reader. The intensity of the colour formed is inversely proportional to the concentration of aldosterone in the sample. A set of standards is used to plot a standard curve from which the amount of aldosterone in patient samples and controls can be directly read.

Typical Standard Curve


Product Manual