Aldosterone ELISA Assay Kit

$230.00

Eagle Biosciences Aldosterone ELISA Assay Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Aldosterone concentration in human serum, human plasma or urine. Aldosterone ELISA Assay Kit is intended for research use only.

SKU: DCM053 Categories: , ,

Aldosterone ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 7 pg/ml
Dynamic Range: 20 – 2000 pg/ml
Incubation Time: 1.5 hour
Sample Type: Serum, Plasma
Sample Size: 25 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

Aldosterone is a steroid hormone produced by the adrenal cortex in the adrenal gland, is the most potent mineralocorticoid in humans, it regulates sodium and potassium balance in the blood.

Aldosterone secretion appears to be stimulated primarily through the renin-angiotensin system. Acting on mineralocorticoid receptors (MR) on principal cells in the collecting ducts of the kidneys, it increases the permeability of their apical (luminal) membrane to potassium and sodium and activates their basolateral Na+/K+ pumps, stimulating ATP hydrolysis, reabsorbing sodium (Na+) ions and water into the blood, and excreting potassium (K+) ions into the urine. Aldosterone regulates plasma bicarbonate (HCO3-) levels and its acid/base balance.

Aldosterone is responsible for the reabsorption of about 2% of filtered sodium in the kidneys.
Plasma aldosterone levels normally vary with body position (upright>supine) and salt intake. Overall plasma aldosterone levels show a circadian rhythm which is similar to but less marked than cortisol, with peak levels in the early morning; about 75% of the daily production is secreted between 04:00 am and 10:00 am each day. Age-related levels tend to decline from fetal through adult life.

Abnormally high plasma aldosterone concentrations can occur in adenomas, glucocorticoid-responsive hyperaldosteronism, idiopathic.

Abnormally low aldosterone secretion occurs in a number of conditions including salt-wasting forms of congenital adrenal hyperplasia, nephropathy, and renal tubular acidosis.

Assay Principle

The principle of this Aldosterone ELISA Assay Kit follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in calibrators, control and patient samples) and an enzyme-labeled antigen (conjugate) for a limited number of antibody binding site on the microwell plate. The washing steps remove unbound materials. After the washing step, the enzyme substrate (TMB) is added. The enzymatic reaction is terminated by addition of the Stop Solution. The intensity of the color is inversely proportional to the concentration of Aldosterone in the sample. The absorbance is measured on a microtiter plate reader. A set of calibrators is used to plot a calibration curve from which the amount of Aldosterone in samples and controls can be directly read.

Manual

Product Manual