Aldosterone ELISA Assay Kit
Aldosterone ELISA Assay Kit is for Research Use Only
Size: 1×96 wells
Sensitivity: 9.1 pg/mL
Dynamic Range: 15–1000 pg/mL
Incubation Time: 80 minutes
Sample Type: Human serum, plasma, or urine
Sample Size: 50 µL
Aldosterone ELISA Assay Kit INTERFERENCE
The following substances were tested and did not show significant interference in the assay:
hemoglobin up to 4 g/L, bilirubin conjugated and free up to 125 mg/L and triglycerides up to 30 mg/mL.
Assay Background for Aldosterone ELISA Assay
Aldosterone is a potent mineralocorticoid whose synthesis and release are controlled by the renin-angiotensin system of the body. Aldosterone promotes the reabsorption of sodium in the distal tubules of the kidney resulting in potassium secretion along with sodium retention, which controls the circulating blood volume. Chronic overproduction and secretion of aldosterone leads to hypertension. Aldosterone measurement levels in serum in conjunction with plasma renin activity levels can be used to differentiate between primary and secondary aldosteronism.
The measurement of aldosterone in concert with selective suppression and stimulation tests can be used to further differentiate primary aldosteronism into two basic types:
• Primary aldosteronism caused by an adenoma of one or both adrenals.
• Primary aldosteronism caused by adrenal hyperplasia.
This differentiation is vital in the treatment and management of the disease. The adrenal adenomas respond well to surgery whereas hyperplastic disease of the adrenals is generally better managed medically. In summary, the precise and accurate measurement of aldosterone by enzyme immunoassay can be an important addition to a diagnostic laboratory battery for the differential diagnosis of hypertensive disease.