Growth Hormone hGH ELISA Assay Kit
The Growth Hormone hGH ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.2 ng/mL
Dynamic Range: 1–50 ng/mL
Incubation Time: 75 minutes
Sample Type: Serum
Sample Size: 25 µL
Alternative Names: Human Growth Hormone ELISA Kit, hGH ELISA
Human growth hormone (hGH) is a polypeptide of 191 amino acids secreted by the somatroph cells of the anterior pituitary. Growth hormone is principally a regulator of body growth and its metabolic effects are primarily anabolic. Some of its effects include promotion of protein conservation through its involvement in a wide range of protein synthesis mechanisms, enhancement of glucose transport and facilitation of glycogen storage. In addition, it induces the release of somatomedins (insulin-like growth factors), which further mediate the cascade of growth promoting actions.
Measurement of hGH is primarily of interest in the diagnosis and treatment of various forms of decreased secretion of hGH. Hyposecretion of hGH in children results in growth retardation and hypersecretion leads to gigantism in children and acromegaly in adults.
The secretion of hGH varies throughout the day under the influence of intricate neurogenic, metabolic and hormonal control. Due to the pulsatile nature of hGH release, it is often inaccurate to define a reference range and status based on single serum measurements. To diagnose disorders of hGH secretion more reliably, dynamic tests are used in which serum hGH levels are measured over a period following suppression or stimulation of hGH secretion.
SPECIMEN COLLECTION AND STORAGE
Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.