Histamine ELISA Assay Kit

$420.00

The Histamine ELISA Assay Kit is intended for the quantitative determination of histamine in Plasma, Urine, and Cell Culture. The Histamine ELISA Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

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Histamine ELISA Assay Kit

Histamine ELISA Assay Kit manufactured in Germany as SKU EA213/96 by DLD Diagnostika

Size: 1×96 wells
Sensitivity: 0.06 ng/ml (plasma), 0.9 ng/ml (urine)
Dynamic Range: 0.15 – 25 ng/ml
Incubation Time: 2.5 hours
Sample Type: Plasma, Urine, Cell Culture
Sample Size: 50 µl
For Research Use Only


Reference Values (Normal)

Normal range plasma:< 1 ng/ml
Normal range urine: < 45 µg/day
It is suggested that each laboratory establish its own normal ranges for the Histamine ELISA

Conversion: 1 ng/ml corresponds to 9.0 nmol/l


Assay Background

Histamine (β-imidazole-ethylamine), a biogenic amine, is a product of the histidine metabolism. It is produced by decarboxylation of histidine. Histamine is widely distributed in mammalian tissues. It’s bound to heparin (as inactive form) and stored in the granules of basophilic leukocytes and mast cells and is actively released as required. These cells, if sensitized by IgE antibodies attached to their membranes, degranulate when exposed to the appropriate antigen.

Histamine plays a major role in the initial phase of an anaphylactic reaction. The quantification of histamine in plasma after allergen administration is of clinical interest. Histamine is part of the immune response to foreign pathogens and it increases the permeability of the capillaries to white blood cells and other proteins in order to allow them to engage foreign invaders in the affected tissues. Responsible for the biological effects of histamine in tissue are the activation of different surface receptors, for instance H1, H2 and H3. Histamine is involved in the regulating physiological function in the gut and acting as a neurotransmitter.


Products Related to Histamine ELISA Assay

High Sensitive Histamine ELISA
Derivatized Histamine ELISA Assay Kit
1-Methylhistamine ELISA Assay Kit


Related News

Click the links below to learn more about studies using this ELISA
Histamine ELISA Assay Kit used in a recent study.
Boston Study: EagleBio’s Histamine ELISA

Additional Information

Assay Principle


The competitive Histamine ELISA Assay kit uses the microtiter plate format. Histamine is bound to the solid phase of the microtiter plate. Acylated histamine and solid phase bound histamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase histamine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase histamine is inversely proportional to the histamine concentration of the sample.

Typical Standard Curve


Histamine ELISA Assay Kit

Manual

Product Manual


Publications

Recent Citations


Ding, Suling, et al. “Histamine Deficiency Aggravates Cardiac Injury through MiR-206/216b-Atg13 Axis-Mediated Autophagic-Dependant Apoptosis.” Cell Death & Disease, vol. 9, no. 6, 7 June 2018, p. 694., doi:10.1038/s41419-018-0723-6.

Zhou, Y;Yu, X;Chen, H;Sjöberg, S;Roux, J;Zhang, L;Ivoulsou, AH;Bensaid, F;Liu, CL;Liu, J;Tordjman, J;Clement, K;Lee, CH;Hotamisligil, GS;Libby, P;Shi, GP.  Leptin Deficiency Shifts Mast Cells toward Anti-Inflammatory Actions and Protects Mice from Obesity and Diabetes by Polarizing M2 Macrophages. Cell Metab. 2015:DOI:10.1016/j.cmet.2015.09.013.

Chen, C;Khismatullin, DB. Oxidized Low-Density Lipoprotein Contributes to Atherogenesis via Co-activation of Macrophages and Mast Cells. Plos One. 2015:DOI: 10.1371/journal.pone.0123088.

Chen, J;Hong, T;Ding, S;Deng, L;Abudupataer, M;Zhang, W;Tong, M;Jia, J;Gong, H;Zou, Y;Wang, TC;Ge, J;Yang, X;, (2017). Aggravated myocardial infarction-induced cardiac remodeling and heart failure in histamine-deficient mice. Sci Rep,7:44007