Histamine ELISA Assay Kit


The Histamine ELISA Assay Kit is intended for the quantitative determination of histamine in Plasma, Whole Blood, Urine, and Cell Culture. The Histamine ELISA Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.
This product was previously known as HIS31-K01.

SKU: EA213/96 Categories: , , Tags: , ,

Histamine ELISA Assay Kit

Histamine ELISA Assay Kit manufactured in Germany as SKU EA213/96 by DLD Diagnostika

Size: 1×96 wells
Sensitivity: 0.06 ng/ml (plasma), 0.9 ng/ml (urine)
Dynamic Range: 0.15 – 25 ng/ml
Incubation Time: 2.5 hours
Sample Type: Plasma (EDTA or Heparin), Whole Blood, Urine and Cell Culture
Sample Size: 50 µl
For Research Use Only

Reference Values (Normal)

Normal range EDTA plasma:< 1 ng/ml
Normal Range Heparin Plasma: <5.4 ng/ml
Normal Range Heparin Whole Blood: 10-100 ng/ml
Normal range urine: < 45 µg/day
It is suggested that each laboratory establish its own normal ranges for the Histamine ELISA

Conversion: 1 ng/ml corresponds to 9.0 nmol/l

Assay Background

Histamine (β-imidazole-ethylamine), a biogenic amine, is a product of the histidine metabolism. It is produced by decarboxylation of histidine. Histamine is widely distributed in mammalian tissues. It’s bound to heparin (as inactive form) and stored in the granules of basophilic leukocytes and mast cells and is actively released as required. These cells, if sensitized by IgE antibodies attached to their membranes, degranulate when exposed to the appropriate antigen.

Histamine plays a major role in the initial phase of an anaphylactic reaction. The quantification of histamine in plasma after allergen administration is of clinical interest. Histamine is part of the immune response to foreign pathogens and it increases the permeability of the capillaries to white blood cells and other proteins in order to allow them to engage foreign invaders in the affected tissues. Responsible for the biological effects of histamine in tissue are the activation of different surface receptors, for instance H1, H2 and H3. Histamine is involved in the regulating physiological function in the gut and acting as a neurotransmitter.

Products Related to Histamine ELISA Assay

High Sensitive Histamine ELISA
Derivatized Histamine ELISA Assay Kit
1-Methylhistamine ELISA Assay Kit

Related News

Click the links below to learn more about studies using this ELISA
Histamine ELISA Assay Kit used in a recent study.
Boston Study: EagleBio’s Histamine ELISA

Additional Information

Assay Principle

The competitive Histamine ELISA Assay kit uses the microtiter plate format. Histamine is bound to the solid phase of the microtiter plate. Acylated histamine and solid phase bound histamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase histamine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase histamine is inversely proportional to the histamine concentration of the sample.

Typical Standard Curve

Histamine ELISA Assay Kit

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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