Serotonin Ultrasensitive ELISA Assay Kit

$530.00

The Eagle Biosciences Serotonin Ultrasensitive ELISA Assay Kit is intended for the quantitative determination of serotonin in biological fluids. The Serotonin Ultrasensitive ELISA Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

SKU: SEU39-K01 Categories: , ,

Serotonin Ultrasensitive ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.39 pg/sample
Dynamic Range: 0.67 – 20 pg/sample
Incubation Time: Overnight
Sample Type: Biological Fluids
Sample Size: 20 µl

Product manufactured in Germany as SKU EA630/96 by DLD Diagnostika

Additional Information

Assay Background

The Eagle Biosciences Serotonin Ultrasensitive ELISA Assay Kit is intended for the quantitative determination of serotonin in biological fluids. The Serotonin Ultrasensitive ELISA Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures. Serotonin (5-Hydroxytryptamine), a biogenic amine, is a product of the tryptophan metabolism. It is a well evaluated neurotransmitter of the central nervous system and can be found in high concentrations in the chromaffine cells of the intestinal mucosa, in the platelets and the serotonergic neurones of the brain. Central-serotonergic neurones influence physiological functions such as sleep and the hormonal and cardio-vascular regulation. Increased serum levels can be found with malignant carcinoid, endogenous depression and schizophrenia.

The Serotonin Ultrasensitive ELISA Assay Kit provides materials for the quantitative measurement of derivated serotonin in low concentrated samples and for small sample volumes. The derivation is performed during the preparation of the samples. By using the acylation reagent, the serotonin is quantitatively derivated into N-acylserotonin.

Assay Principle

The competitive Serotonin Ultrasensitive ELISA Assay kit uses the microtiter plate format. Derivated serotonin competes for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase serotonin is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase serotonin is inversely proportional to the serotonin concentration of the sample.


Typical Standard Curve

Serotonin Sensitive ELISA Assay Standard Curve

Manual

Product Manual


Publications

Citations

Utilizing Mouse/Rat Samples:

  • Malkova, NV;Gallagher, JJ;Yu, CZ;Jacobs, RE;Patterson, PH; Manganese-enhanced magnetic resonance imaging reveals increased DOI-induced brain activity in a mouse model of schizophrenia, Proc. Natl. Acad. Sci. U.S.A., 2014, Volume 111, Issue 24, Page E2492-500