Rat Legumain ELISA Kit


The Rat Legumain ELISA Kit is intended for the quantitation of Rat LGMN concentrations in cell culture supernates, serum and plasma (heparin, EDTA). The Eagle Biosciences Rat Legumain ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: RLG21-K01 Categories: , ,

Rat Legumain ELISA Kit

The Rat Legumain ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 31.2pg/ml-2000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Sample Size: 100 µl
Alternative Name: LGMN

Assay Principle

The Rat Lgmn Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Lgmn with a 96-well strip plate that is pre-coated with antibody specific for Lgmn. The detection antibody is a biotinylated antibody specific for Lgmn. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Lgmn with immunogen: Expression system for standard: NSO, Immunogen sequence: V18-N325. The kit is analytically validated with ready to use reagents. To measure Rat Lgmn, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Lgmn in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Rat Lgmn in the sample.

Related Products

Human Total Legumain ELISA Kit
Mouse Total Legumain ELISA Kit

Additional Information

Assay Background

Legumain is a protein that in humans is encoded by the LGMN gene. It is mapped to 6q32. This gene encodes a cysteine protease, legumain, that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.

Typical Standard Curve

Rat Legumain ELISA Kit


Product Documents


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