Human Kallikrein-6 ELISA Kit

$515.00

The Human Kallikrein-6 ELISA Kit is intended for the quantitation of Human KLK6 concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human Kallikrein-6 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: KL631-K01 Categories: , ,

Human Kallikrein-6 ELISA Kit

The Human Kallikrein-6 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <3pg/ml
Standard Range: 78pg/ml-5,000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: cell culture supernates, cell lysates, serum and plasma(heparin, EDTA)
Sample Size: 100 µl
Alternative Names: Kallikrein-related peptidase 6, Kallikrein 6, Neurosin, Protease M, ZYME or PRSS9


Assay Principle

The Human KLK6 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human KLK6 with a 96-well strip plate that is pre-coated with antibody specific for KLK6. The detection antibody is a biotinylated antibody specific for KLK6. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human KLK6 with immunogen: Expression system for standard: NSO; Immunogen sequence: L22-K244. The kit is analytically validated with ready to use reagents. To measure Human KLK6, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human KLK6 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human KLK6 in the sample.


Related Products

Human KLK1 ELISA Kit
Human KLK5 ELISA Kit
Human Kallikrein-3 ELISA Kit

Additional Information

Assay Background


KLK6 (Kallikrein-related peptidase 6), also called KALLIKREIN 6, NEUROSIN, PROTEASE M, ZYME or PRSS9, is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. Northern blot analysis revealed that the PRSS9 mRNA was expressed in several primary tumors and cell lines from mammary, prostate, and ovarian cancers, but was not detected in any metastases of these cancers. The KLK6 gene is mapped on 19q13.41. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer’s disease. Upon cellular stress, neurosin was released from mitochondria to the cytosol, which resulted in the increase of degraded alpha-synuclein species. Neurosin may play a significant role in physiologic alpha-synuclein degradation and also in the pathogenesis of synucleinopathies.

Typical Standard Curve


Documents

Product Documents


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations