The Mouse LIF ELISA Kit is intended for the quantitation of Mouse LIF concentrations in cell culture supernates, serum and plasma (heparin, EDTA). The Eagle Biosciences Mouse LIF ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.


The Mouse LIF ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 7.8pg/ml-500pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA)
Sample Size: 100 µl
Alternative name: Leukemia inhibitory factor
Storage Instructions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freezethaw cycles (Shipped with wet ice.)

Assay Background

Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.

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Additional Information

Assay Principle

The Mouse Lif Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Lif with a 96-well strip plate that is pre-coated with antibody specific for Lif. The detection antibody is a biotinylated antibody specific for Lif. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Lif with immunogen: Expression system for standard: E.coli; Immunogen sequence: S24-F203. The kit is analytically validated with ready to use reagents. To measure Mouse Lif, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Lif in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse Lif in the sample.

Typical Standard Curve



Product Documents


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