Human Total Legumain ELISA Kit
The Human Total Legumain ELISA Kit is For Research Use Only
Size: 1×96 wells
Standard Range: 31.2pg/ml-2000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Sample Size: 100 µl
Alternative Names: LGMN, Asparaginyl Endopeptidase, Citvac, Proteinase B, Hemoglobinase, PRSC1 , Vicilin Peptidohydrolase, Bean Endopeptidase
The Human LGMN Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human LGMN with a 96-well strip plate that is pre-coated with antibody specific for LGMN. The detection antibody is a biotinylated antibody specific for LGMN. The kit contains recombinant Human LGMN with immunogen: Expression system for standard: NSO; Immunogen sequence: V18-Y433. The kit is analytically validated with ready to use reagents. To measure Human LGMN, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human LGMN in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human LGMN in the sample.