Mouse Total Legumain ELISA Kit


The Mouse Total Legumain ELISA Kit is intended for the quantitation of Mouse LGMN concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Mouse Legumain (total) ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

Mouse Total Legumain ELISA Kit

The Mouse Total Legumain ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 31.2pg/ml-2000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Sample Size: 100 µl
Alternative Names: Asparaginyl Endopeptidase, Citvac, Proteinase B, Hemoglobinase, PRSC1 gene product, Vicilin Peptidohydrolase, Bean Endopeptidase

Assay Background

The Mouse Lgmn Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Lgmn with a 96-well strip plate that is pre-coated with antibody specific for Lgmn. The detection antibody is a biotinylated antibody specific for Lgmn. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Lgmn with immunogen: Expression system for standard: NSO; Immunogen sequence: V18-Y435. The kit is analytically validated with ready to use reagents. To measure Mouse Lgmn, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Lgmn in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse Lgmn in the sample.

Related Products

Rat Legumain ELISA Kit
Human Total Legumain ELISA Kit

Additional Information

Assay Background

Legumain (asparaginyl endopeptidase, citvac, proteinase B, hemoglobinase, PRSC1 gene product or LGMN (Homo sapiens), vicilin peptidohydrolase, bean endopeptidase) is an enzyme that in humans is encoded by the LGMN gene (previous symbolPRSC1). Using fluorescence in situ hybridization, the LGMN gene was mapped to chromosome 14q32.1. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types.

Typical Standard Curve

Mouse Total Legumain ELISA Kit


Product Manual


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