Rat GLP-2 ELISA Assay
The Rat GLP-2 ELISA Assay is For Research Use Only
Size: 1×96 wells
Dynamic Range: 0.137 – 100 ng/mL
Incubation Time: 20 hours
Sample Type: Rat Serum, Plasma
Sample Size: 25 µL
Alternative Name: Glucagon-like Peptide 2, Rodent GLP-2
Specificity: This Rat GLP-2 ELISA Assay kit has high specificity to rat GLP-2 and shows no cross reactivity with rat glucagon and rat GLP-1 even in the concentration of 300 pmol/mL.
This Rat GLP-2 ELISA for determination of rat GLP-2 in serum or plasma samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to rat GLP-2 and biotin-avidin affinity system. The 96-wells plate is coated with goat anti rabbit IgG antibody. Rat GLP-2 standard or samples, labeled antigen and anti-rat GLP-2 polyclonal antibody are added to the wells for competitive immunoreaction. After incubation and plate washing, HRP labeled streptoavidin (SA-HRP) are added to form HRP labeled streptoavidin-biotinylated rat GLP-2-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of rat GLP-2 is calculated.