Vitamin ELISA Assay

Pantothenic Acid Microtiter Plate Assay Kit

$1,180.00

The Pantothenic Acid Assay Kit is a microtiter plate test kit based on a microbiological assay which measures the total pantothenic acid content in serum. The test kit contains all required reagents, e.g.standard, medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the pantothenic acid content. The Pantothenic Acid Assay kit is For Research Use Only.

SKU: KIFR004 Categories: , ,

Pantothenic Acid Microtiter Plate Assay Kit

Pantothenic Acid Microtiter Plate Assay Kit manufactured in Germany by Immundiagnostik

Size: 1×96 wells
Sensitivity: 2.3 µg/L
Dynamic Range: 2.3 – 36.8 µg/L
Incubation Time: 48 hours
Sample Type: Serum
Sample Size: 100 µL
Alternative Names: Vitamin B5
For Research Use Only
Controls Included

Assay Background

Pantothenic acid (vitamin B5) is synthesized by most microorganisms and plants from pantoic acid. The vitamin is an integral part of 4-phosphopantheine, which is a component of coenzyme A (CoA). CoA plays a key role in the metabolism of numerous compounds, especially lipids and the ultimate catabolic disposition of carbohydrates and ketogenic amino acids. About 80% of the vitamin in animal tissues is in CoA form, and the rest exists mainly as phosphopantetheine and phosphopantethenate.

Another essential role of pantothenic acid is its participation in the 4-phosphopantetheine moiety of acyl carrier protein (ACP), where the phosphodiester-linked prosthetic group uses the sulfhydryl terminus to exchange with malonyl-CoAto form an ACP-Smalonyl thioester, which can chain elongate during fatty acid biosynthesis.

Pantothenic acid deficiency is exceedingly rare. Because of its rarity, most information about pantothenic acid deficiency has been obtained from experiments: Pantothenic acid deficiency has been induced in humans by use of a metabolic antagonist, w-methyl pantothenic acid along with a pantothenic acid-deficient diet. Subjects became irascible and developed postural hypotension and rapid heart rate on exertion, epigastric distress with anorexia and constipation, numbness and tingling of the hands and feet. Because pantothenic acid is involved with so many vital processes in the body, it is not surprising that a broad number of complications might result from deficiency.

From recent research, it is known that the pantothenic acid derivative, pantethine (two molecules of pantetheine joined by a disulfide bond), has a hypocholesterolemic effect. A metabolic antagonist of pantothenic acid, pantoyl g-aminobutyric acid (called pantoyl-GABA), is widely used in Japan as an anti-dementia drug for treating cognitive impairments in pathological states such as Alzheimer‘s disease, presumably through increasing cholinergic activity in vivo.

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Additional Information

Assay Principle

Serum samples are diluted and added into the microtiter plate wells coated with Lactobacillus plantarum which metabolizes pantothenic acid. The presence of pantothenic acid both in standards [STD] and samples gives a pantothenic acid-dependent growth response. After incubation at 37°C for 24 hours, the growth of Lactobacillus plantarum is measured turbidimetrically at 610 – 630 nm (alternative at 540 – 550 nm) in an ELISA-reader. A dose-response curve of absorbance unit (optical density, OD at 610 nm) vs. concentration is generated using the values obtained from the standard. Pantothenic acid present in the patient samples is determined directly from this curve.

Manual

Product Manual