Mouse IgE ELISA Assay Kit

$380.00

The Eagle Biosciences Mouse IgE ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgE in biological samples of mice.

Mouse IgE ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 1.911 ng/ml
Dynamic Range: 6.25 ng/ml – 400 ng/ml
Incubation Time: 70 minutes
Sample Type: Serum, Plasma
Sample Size: 100 µl

Product manufactured in the USA

Additional Information

Assay Principle


The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgE present in samples reacts with the anti-IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgE in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Typical Standard Curve


Manual

Product Manual