Rat GIP ELISA Assay
THe Rat GIP ELISA Assay is For Research Use Only
Size: 1×96 wells
Dynamic Range: 3.9 – 250 pg/mL
Incubation Time: 3.5 hours
Sample Type: Rat Plasma, Culture medium supernatant
Sample Size: 50μL
Specificity: This assay kit has high specificity to rat GIP (1-42) active form and shows no cross reactivity to rat GIP (3-42) inactive form, glucagons, rat GLP-2, GLP-1 (7-36) NH2 and GLP-1 (9-36) NH2.
Alternative Names: Rat glucose-dependent insulinotropic polypeptide, Rat GIP 1-42, Rat Active GIP
This Rat GIP (Active) ELISA for determination of rat GIP (1-42) active form is based on a sandwich enzyme immunoassay. To the wells of plate coated with highly purified mouse monoclonal antibody against rat GIP (1-42) active form, standards or samples are added for the 1st step immunoreaction. After the 1st step incubation and plate washing, HRP labeled antibody solution against rat GIP (1-42) is added as the 2nd step to form antibody – antigen – labeled antibody complex on the surface of the wells. After the 2nd step incubation and rinsing out excess labeled antibody, Finally, HRP enzyme activity is determined by 3,3’,5,5’-Tetramethylbenzidine (TMB) and the concentration of rat GIP (1-42) active form is calculated.