The Rat FLT4 ELISA Kit is intended for the quantitation of Rat FLT4 concentrations in cell culture supernates and serum. The Eagle Biosciences Rat FLT4/VEGFR3 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: FT421-K01 Categories: , ,


The Rat FLT4 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 156pg/ml-10000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates and Serum
Sample Size: 100 µl
Alternative Names: Fms-related tyrosine kinase 4, VEGFR3

Assay Principle

The Rat FLT4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat FLT4 with a 96-well strip plate that is pre-coated with antibody specific for FLT4. The detection antibody is a biotinylated antibody specific for FLT4. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat FLT4 with immunogen: Expression system for standard: NSO; Immunogen sequence: Y25-D770. The kit is analytically validated with ready to use reagents. To measure Rat FLT4, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat FLT4 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Rat FLT4 in the sample.

Related Products

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Additional Information

Assay Background

Fms-related tyrosine kinase 4, also known as FLT4, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.

Typical Standard Curve



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