The GDF-15 ELISA Assay Kit is designed for quantification of human GDF-15 in serum, plasma and cell culture supernate samples. The Eagle Biosciences GDF-15 ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

SKU: GDF31-K01 Categories: , ,

GDF-15 ELISA Assay Kit

The GDF-15 ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 3.9pg/ml
Standard Range: 7.8pg/ml-500pg/ml
Incubation Time: 2 hours 35 minutes
Sample Type: Serum and Plasma
Sample Size: 100 µl
Alternative Names: Growth differentiation factor 15, TGF-PL, MIC-1, PDF, PLAB, and PTGFB

Assay Principle

The GDF-15 assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human GDF-15. Standards and samples are pipetted into the wells and any human GDF-15 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human GDF-15 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and color develops in proportion to the amount of human GDF-15 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human GDF-15, the unknown sample concentration can be interpolated from a reference curve included in each assay.

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Additional Information

Assay Background

Growth differentiation factor 15 (GDF-15) is a member of the transforming growth factor β cytokine superfamily, the protein is secreted as a 25 kDa disulfide linked dimer. Circulating GDF-15 levels are associated with cancers, cardiovascular and kidney diseases. GDF-15 regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.


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