Mouse FLT4/VEGFR3 ELISA Kit

$515.00

The Mouse FLT4/VEGFR3 ELISA Kit is intended for the quantitation of Mouse FLT4 concentrations in cell culture supernates and serum. The Eagle Biosciences Mouse FLT4/VEGFR3 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: FT411-K01 Categories: , ,

Mouse FLT4/VEGFR3 ELISA Kit

The Mouse FLT4/VEGFR3 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 156pg/ml-10,000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates and Serum
Sample Size: 100 µl


Assay Principle

The Mouse FLT4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse FLT4 with a 96-well strip plate that is pre coated with antibody specific for FLT4. The detection antibody is a biotinylated antibody specific for FLT4. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse FLT4 with immunogen: Expression system for standard: NSO; Immunogen sequence: Y25-D770. The kit is analytically validated with ready to use reagents. To measure Mouse FLT4, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse FLT4 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse FLT4 in the sample.


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Additional Information

Assay Background


Fms-related tyrosine kinase 4, also known as FLT4, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.

Typical Standard Curve


Mouse FLT4/VEGFR3 ELISA Kit

Documents

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Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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